Troponin I Type 3, Cardiac (TNNI3)

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cTnI; TNNC1; CMH7; C-TnI; Troponin I Type 3; Cardiac troponin I

Troponin I Type 3, Cardiac (TNNI3)

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA478Hu01Recombinant Troponin I Type 3, Cardiac (TNNI3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA478Hu01Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
MAA478Hu22Monoclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
LAA478Hu71Biotin-Linked Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)IHC;WB;ELISA
Assay KitsSEA478HuELISA Kit for Troponin I Type 3, Cardiac (TNNI3)Enzyme-linked immunosorbent assay for Antigen Detection
SCA478HuCLIA Kit for Troponin I Type 3, Cardiac (TNNI3)Chemiluminescent immunoassay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA478Mu01Recombinant Troponin I Type 3, Cardiac (TNNI3)SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
RPA478Mu02Recombinant Troponin I Type 3, Cardiac (TNNI3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA478Mu01Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
LAA478Mu71Biotin-Linked Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)IHC;WB;ELISA
LAA478Mu81FITC-Linked Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)IHC;ICC;IF;WB
PAA478Mu02Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA478MuELISA Kit for Troponin I Type 3, Cardiac (TNNI3)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA478Ra01Recombinant Troponin I Type 3, Cardiac (TNNI3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA478Ra01Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
MAA478Ra21Monoclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA478RaELISA Kit for Troponin I Type 3, Cardiac (TNNI3)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA478Rb01Recombinant Troponin I Type 3, Cardiac (TNNI3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA478Rb51Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
PAA478Rb52Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA478RbELISA Kit for Troponin I Type 3, Cardiac (TNNI3)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA478Po01Recombinant Troponin I Type 3, Cardiac (TNNI3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA478Po01Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
MAA478Po21Monoclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA478PoELISA Kit for Troponin I Type 3, Cardiac (TNNI3)Enzyme-linked immunosorbent assay for Antigen Detection
  1. "Molecular cloning of human cardiac troponin I using polymerase chain reaction."FEBS Lett. 270:57-61(1990) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning and expression in Escherichia coli of the cDNA encoding human cardiac troponin I."Gene 131:287-292(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Troponin I isoform expression in human heart."Circ. Res. 69:1409-1414(1991) [PubMed] [Europe PMC] [Abstract]
  4. "Isolation and characterization of the human cardiac troponin I gene (TNNI3)."Genomics 35:11-23(1996) [PubMed] [Europe PMC] [Abstract]
  5. "A common motif of two adjacent phosphoserines in bovine, rabbit and human cardiac troponin I."FEBS Lett. 273:41-45(1990) [PubMed] [Europe PMC] [Abstract]
  6. "The ordered phosphorylation of cardiac troponin I by the cAMP-dependent protein kinase -- structural consequences and functional implications."Eur. J. Biochem. 248:329-337(1997) [PubMed] [Europe PMC] [Abstract]
  7. "p21-activated kinase increases the calcium sensitivity of rat triton-skinned cardiac muscle fiber bundles via a mechanism potentially involving novel phosphorylation of troponin I."Circ. Res. 91:509-516(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Protein kinase D is a novel mediator of cardiac troponin I phosphorylation and regulates myofilament function."Circ. Res. 95:1091-1099(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I."Proc. Natl. Acad. Sci. U.S.A. 101:18135-18140(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation of cardiac troponin I by mammalian sterile 20-like kinase 1."Biochem. J. 418:93-101(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Multiple reaction monitoring to identify site-specific troponin I phosphorylated residues in the failing human heart."Circulation 126:1828-1837(2012) [PubMed] [Europe PMC] [Abstract]
  12. "Binding of cardiac troponin-I147-163 induces a structural opening in human cardiac troponin-C."Biochemistry 38:8289-8298(1999) [PubMed] [Europe PMC] [Abstract]
  13. "Structure of the regulatory N-domain of human cardiac troponin C in complex with human cardiac troponin I147-163 and bepridil."J. Biol. Chem. 277:31124-31133(2002) [PubMed] [Europe PMC] [Abstract]
  14. "Mutations in the cardiac troponin I gene associated with hypertrophic cardiomyopathy." Nat. Genet. 16:379-382(1997) [PubMed] [Europe PMC] [Abstract]
  15. "Sarcomere protein gene mutations in hypertrophic cardiomyopathy of the elderly."Circulation 105:446-451(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Idiopathic restrictive cardiomyopathy is part of the clinical expression of cardiac troponin I mutations."J. Clin. Invest. 111:209-216(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Hypertrophic cardiomyopathy: distribution of disease genes, spectrum of mutations, and implications for a molecular diagnosis strategy."Circulation 107:2227-2232(2003) [PubMed] [Europe PMC] [Abstract]
  18. ErratumCirculation 109:3258-3258(2004)
  19. "Mutation spectrum in a large cohort of unrelated consecutive patients with hypertrophic cardiomyopathy."Clin. Genet. 64:339-349(2003) [PubMed] [Europe PMC] [Abstract]
  20. "Novel mutation in cardiac troponin I in recessive idiopathic dilated cardiomyopathy."Lancet 363:371-372(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Compound and double mutations in patients with hypertrophic cardiomyopathy: implications for genetic testing and counselling."J. Med. Genet. 42:E59-E59(2005) [PubMed] [Europe PMC] [Abstract]
  22. "Identification and functional characterization of cardiac troponin I as a novel disease gene in autosomal dominant dilated cardiomyopathy."Circ. Res. 105:375-382(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Clinical and mutational spectrum in a cohort of 105 unrelated patients with dilated cardiomyopathy."Eur. J. Med. Genet. 54:E570-E575(2011) [PubMed] [Europe PMC] [Abstract]