Apolipoprotein A1 (APOA1)

[Edit]

Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I

Apolipoprotein A1 (APOA1)

Apolipoprotein A-I  the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters.

ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.ApoA-1 Milano is a naturally occurring mutant of ApoA-I, found in a family descended from a single couple of the 18th century.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA519Hu01Recombinant Apolipoprotein A1 (APOA1)SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
AntibodiesPAA519Hu01Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
MAA519Hu22Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA519Hu71Biotin-Linked Polyclonal Antibody to Apolipoprotein A1 (APOA1)IHC;WB;ELISA
MAA519Hu21Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
MAA519Hu24Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
MAA519Hu26Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA519Hu76Biotin-Linked Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; IF; ELISA.
Assay KitsSEA519HuELISA Kit for Apolipoprotein A1 (APOA1)Enzyme-linked immunosorbent assay for Antigen Detection
SCA519HuCLIA Kit for Apolipoprotein A1 (APOA1)Chemiluminescent immunoassay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA519Mu01Recombinant Apolipoprotein A1 (APOA1)SDS-PAGE; WB; ELISA; IP.
RPA519Mu02Recombinant Apolipoprotein A1 (APOA1)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA519Mu01Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
LAA519Mu71Biotin-Linked Polyclonal Antibody to Apolipoprotein A1 (APOA1)IHC;WB;ELISA
LAA519Mu81FITC-Linked Polyclonal Antibody to Apolipoprotein A1 (APOA1)IHC;ICC;IF;WB
PAA519Mu02Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA519MuELISA Kit for Apolipoprotein A1 (APOA1)Enzyme-linked immunosorbent assay for Antigen Detection
SCA519MuCLIA Kit for Apolipoprotein A1 (APOA1)Chemiluminescent immunoassay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA519Ra01Recombinant Apolipoprotein A1 (APOA1)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA519Ra01Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA519Ra71Biotin-Linked Polyclonal Antibody to Apolipoprotein A1 (APOA1)IHC;WB;ELISA
MAA519Ra21Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
Assay KitsSEA519RaELISA Kit for Apolipoprotein A1 (APOA1)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA519Rb01Recombinant Apolipoprotein A1 (APOA1)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA519Rb51Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
MAA519Rb21Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA519Rb71Biotin-Linked Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; IF; ELISA.
Assay KitsCEA519RbELISA Kit for Apolipoprotein A1 (APOA1)Enzyme-linked immunosorbent assay for Antigen Detection
SEA519RbELISA Kit for Apolipoprotein A1 (APOA1)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA519Si01Recombinant Apolipoprotein A1 (APOA1)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA519Si01Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
MAA519Si21Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA519SiELISA Kit for Apolipoprotein A1 (APOA1)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA519Po01Recombinant Apolipoprotein A1 (APOA1)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA519Po01Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA519Po81FITC-Linked Polyclonal Antibody to Apolipoprotein A1 (APOA1)IHC;ICC;IF;WB
LAA519Po71Biotin-Linked Polyclonal Antibody to Apolipoprotein A1 (APOA1)IHC;WB;ELISA
MAA519Po21Monoclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA519PoELISA Kit for Apolipoprotein A1 (APOA1)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Apolipoprotein A1 (APOA1)Recombinant Protein Customized Service Offer
AntibodiesPAA519Bo01Polyclonal Antibody to Apolipoprotein A1 (APOA1)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Apolipoprotein A1 (APOA1)CLIA Kit Customized Service Offer
n/aELISA Kit for Apolipoprotein A1 (APOA1)ELISA Kit Customized Service Offer
  1. "Gene structure of human apolipoprotein A1."Nucleic Acids Res. 11:2827-2837(1983) [PubMed] [Europe PMC] [Abstract]
  2. "Nucleotide sequence of cloned cDNA of human apolipoprotein A-I."Nucleic Acids Res. 11:3703-3715(1983) [PubMed] [Europe PMC] [Abstract]
  3. "Isolation and characterization of the human apolipoprotein A-I gene."Proc. Natl. Acad. Sci. U.S.A. 80:6147-6151(1983) [PubMed] [Europe PMC] [Abstract]
  4. "Isolation and DNA sequence of full-length cDNA and of the entire gene for human apolipoprotein AI -- discovery of a new genetic polymorphism in the apo AI gene."DNA 3:309-317(1984) [PubMed] [Europe PMC] [Abstract]
  5. "Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance."Nucleic Acids Res. 12:3917-3932(1984) [PubMed] [Europe PMC] [Abstract]
  6. "Nucleotide sequence and the encoded amino acids of human apolipoprotein A-I mRNA."Proc. Natl. Acad. Sci. U.S.A. 81:66-70(1984) [PubMed] [Europe PMC] [Abstract]
  7. "Tangier disease. The complete mRNA sequence encoding for preproapo-A-I."J. Biol. Chem. 260:12810-12814(1985) [PubMed] [Europe PMC] [Abstract]
  8. "Sequence and expression of Tangier apoA-I gene."Eur. J. Biochem. 173:465-471(1988) [PubMed] [Europe PMC] [Abstract]
  9. "Production of human recombinant proapolipoprotein A-I in Escherichia coli: purification and biochemical characterization."DNA 8:429-436(1989) [PubMed] [Europe PMC] [Abstract]
  10. "The effects of scale: variation in the APOA1/C3/A4/A5 gene cluster."Hum. Genet. 115:36-56(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Expression of human proapolipoprotein A-1."Patent number EP0293357, 30-NOV-1988
  12. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  13. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Human plasma proapoA-I: isolation and amino-terminal sequence."Biochem. Biophys. Res. Commun. 113:626-632(1983) [PubMed] [Europe PMC] [Abstract]
  15. "The primary structure of human plasma high density apolipoprotein glutamine I (ApoA-I). II. The amino acid sequence and alignment of cyanogen bromide fragments IV, III, and I."J. Biol. Chem. 250:2725-2738(1975) [PubMed] [Europe PMC] [Abstract]
  16. "The amino acid sequence of human APOA-I, an apolipoprotein isolated from high density lipoproteins."Biochem. Biophys. Res. Commun. 80:623-630(1978) [PubMed] [Europe PMC] [Abstract]
  17. "Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I). A novel function of Apo A-I."J. Clin. Invest. 82:803-807(1988) [PubMed] [Europe PMC] [Abstract]
  18. "Identification of apolipoprotein A1 and immunoglobulin as components of a serum complex that mediates activation of human sperm motility."Biochemistry 30:8986-8990(1991) [PubMed] [Europe PMC] [Abstract]
  19. "Apolipoprotein A-I binds to a family of bovine seminal plasma proteins."J. Biol. Chem. 264:16853-16857(1989) [PubMed] [Europe PMC] [Abstract]
  20. "Similarity of cruzin, an inhibitor of Trypanosoma cruzi neuraminidase, to high-density lipoprotein."Science 238:1417-1419(1987) [PubMed] [Europe PMC] [Abstract]
  21. "The human myocardial two-dimensional gel protein database: update 1994."Electrophoresis 15:1459-1465(1994) [PubMed] [Europe PMC] [Abstract]
  22. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
  23. "The apolipoprotein A-I binding protein of placenta and the SP-40,40 protein of human blood are different proteins which both bind to apolipoprotein A-I."Biochim. Biophys. Acta 1086:255-260(1991) [PubMed] [Europe PMC] [Abstract]
  24. , Submitted (DEC-2008) to UniProtKB
  25. "Isolation and characterization of cDNA clones for human apolipoprotein A-I."Proc. Natl. Acad. Sci. U.S.A. 79:6861-6865(1982) [PubMed] [Europe PMC] [Abstract]
  26. "Human apolipoprotein A-I. Post-translational modification by fatty acid acylation."J. Biol. Chem. 261:3911-3914(1986) [PubMed] [Europe PMC] [Abstract]
  27. "Intracellular and extracellular processing of human apolipoprotein A-I: secreted apolipoprotein A-I isoprotein 2 is a propeptide."Proc. Natl. Acad. Sci. U.S.A. 80:2574-2578(1983) [PubMed] [Europe PMC] [Abstract]
  28. "The preferential site of non-enzymatic glycation of human apolipoprotein A-I in vivo."Clin. Chim. Acta 217:193-198(1993) [PubMed] [Europe PMC] [Abstract]
  29. "Cloning and characterization of a novel apolipoprotein A-I-binding protein, AI-BP, secreted by cells of the kidney proximal tubules in response to HDL or ApoA-I."Genomics 79:693-702(2002) [PubMed] [Europe PMC] [Abstract]
  30. "Characterization of specifically oxidized apolipoproteins in mildly oxidized high density lipoprotein."J. Lipid Res. 44:349-355(2003) [PubMed] [Europe PMC] [Abstract]
  31. "Novel mass spectrometric immunoassays for the rapid structural characterization of plasma apolipoproteins."J. Lipid Res. 44:630-639(2003) [PubMed] [Europe PMC] [Abstract]
  32. "NDRG1 interacts with APO A-I and A-II and is a functional candidate for the HDL-C QTL on 8q24."Biochem. Biophys. Res. Commun. 332:982-992(2005) [PubMed] [Europe PMC] [Abstract]
  33. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  34. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  35. "Secondary structure changes in ApoA-I Milano (R173C) are not accompanied by a decrease in protein stability or solubility."PLoS ONE 9:E96150-E96150(2014) [PubMed] [Europe PMC] [Abstract]
  36. "Conformation of human serum apolipoprotein A-I(166-185) in the presence of sodium dodecyl sulfate or dodecylphosphocholine by 1H-NMR and CD. Evidence for specific peptide-SDS interactions."Biochim. Biophys. Acta 1301:174-184(1996) [PubMed] [Europe PMC] [Abstract]
  37. "Crystal structure of truncated human apolipoprotein A-I suggests a lipid-bound conformation."Proc. Natl. Acad. Sci. U.S.A. 94:12291-12296(1997) [PubMed] [Europe PMC] [Abstract]
  38. "Autosomal dominant hypoalphalipoproteinemia due to a completely defective apolipoprotein A-I gene."Biochem. Biophys. Res. Commun. 196:950-955(1993) [PubMed] [Europe PMC] [Abstract]
  39. "Apolipoprotein A-I Q[-2]X causing isolated apolipoprotein A-I deficiency in a family with analphalipoproteinemia."J. Clin. Invest. 93:223-229(1994) [PubMed] [Europe PMC] [Abstract]
  40. "Apolipoprotein A-IMilano. Detection of normal A-I in affected subjects and evidence for a cysteine for arginine substitution in the variant A-I."J. Biol. Chem. 258:2508-2513(1983) [PubMed] [Europe PMC] [Abstract]
  41. "Tangier disease: defective recombination of a specific Tangier apolipoprotein A-I isoform (pro-apo A-I) with high density lipoproteins."Proc. Natl. Acad. Sci. U.S.A. 80:6081-6085(1983) [PubMed] [Europe PMC] [Abstract]
  42. "Apolipoprotein A-IGiessen (Pro143-->Arg). A mutant that is defective in activating lecithin:cholesterol acyltransferase."Eur. J. Biochem. 144:325-331(1984) [PubMed] [Europe PMC] [Abstract]
  43. "Abnormal lecithin:cholesterol acyltransferase activation by a human apolipoprotein A-I variant in which a single lysine residue is deleted."J. Biol. Chem. 259:10063-10070(1984) [PubMed] [Europe PMC] [Abstract]
  44. "Variant apolipoprotein AI as a major constituent of a human hereditary amyloid."Biochem. Biophys. Res. Commun. 156:762-768(1988) [PubMed] [Europe PMC] [Abstract]
  45. "A mutation in apolipoprotein A-I in the Iowa type of familial amyloidotic polyneuropathy."Genomics 8:318-323(1990) [PubMed] [Europe PMC] [Abstract]
  46. "Isolation and characterization of human apolipoprotein A-I Fukuoka (110 Glu-->Lys). A novel apolipoprotein variant."Biochim. Biophys. Acta 1043:169-176(1990) [PubMed] [Europe PMC] [Abstract]
  47. "Apolipoprotein AI mutation Arg-60 causes autosomal dominant amyloidosis."Proc. Natl. Acad. Sci. U.S.A. 89:7389-7393(1992) [PubMed] [Europe PMC] [Abstract]
  48. "Apolipoprotein A1 Baltimore (Arg-10-->Leu), a new ApoA1 variant."Hum. Genet. 84:439-445(1990) [PubMed] [Europe PMC] [Abstract]
  49. "Apolipoprotein A-I variants. Naturally occurring substitutions of proline residues affect plasma concentration of apolipoprotein A-I."J. Clin. Invest. 84:1722-1730(1989) [PubMed] [Europe PMC] [Abstract]
  50. "Structural analysis of human apolipoprotein A-I variants. Amino acid substitutions are nonrandomly distributed throughout the apolipoprotein A-I primary structure."J. Biol. Chem. 265:8610-8617(1990) [PubMed] [Europe PMC] [Abstract]
  51. "Characterization of two new human apolipoprotein A-I variants: apolipoprotein A-I Tsushima (Trp-108-->Arg) and A-I Hita (Ala-95-->Asp)."Biochim. Biophys. Acta 1214:272-278(1994) [PubMed] [Europe PMC] [Abstract]
  52. "Familial nephropathic systemic amyloidosis caused by apolipoprotein AI variant Arg26."Q. J. Med. 87:149-154(1994) [PubMed] [Europe PMC] [Abstract]
  53. "A novel homozygous missense mutation in the apo A-I gene with apo A-I deficiency."Arterioscler. Thromb. Vasc. Biol. 18:389-396(1998) [PubMed] [Europe PMC] [Abstract]
  54. "Apo A-I Zaragoza(L144R): a novel mutation in the apolipoprotein A-I gene associated with familial hypoalphalipoproteinemia."Hum. Mutat. 11:416-416(1998)
  55. "Association of extreme blood lipid profile phenotypic variation with 11 reverse cholesterol transport genes and 10 non-genetic cardiovascular disease risk factors."Hum. Mol. Genet. 12:2733-2743(2003) [PubMed] [Europe PMC] [Abstract]
  56. "Quantitative detection of single amino acid polymorphisms by targeted proteomics."J. Mol. Cell Biol. 3:309-315(2011) [PubMed] [Europe PMC] [Abstract]