Protein Kinase R (PKR)

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PRKR; PRK-R; EIF2AK2; EIF2AK1; Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; P1/eIF-2A kinase

Protein Kinase R (PKR)

PKR is activated by double-stranded RNA (dsRNA), the synthesis of which is caused virally. PKR can also be activated by the protein PACT or by heparin. PKR contains an N-terminal dsRNA binding domain (dsRBD) and a C-terminal kinase domain, that gives it pro-apoptotic (cell-killing) functions. The dsRBD consists of two tandem copies of a conserved double stranded RNA binding motif, dsRBM1 and dsRBM2. PKR is induced by interferon in a latent state. Binding to dsRNA is believed to activate PKR by inducing dimerization and subsequent autophosphorylation reactions. In situations of viral infection, the dsRNA created by viral replication and gene expression binds to the N-terminal domain, activating the protein and causing apoptosis of the cell to prevent further viral spread.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA520Hu01Recombinant Protein Kinase R (PKR)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA520Hu01Polyclonal Antibody to Protein Kinase R (PKR)WB, ICC, IHC-P, IHC-F, ELISA
MAA520Hu22Monoclonal Antibody to Protein Kinase R (PKR)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA520HuELISA Kit for Protein Kinase R (PKR)Enzyme-linked immunosorbent assay

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA520Mu01Recombinant Protein Kinase R (PKR)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA520Mu01Polyclonal Antibody to Protein Kinase R (PKR)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Protein Kinase R (PKR)CLIA Kit Customized Service Offer
n/aELISA Kit for Protein Kinase R (PKR)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA520Ra01Recombinant Protein Kinase R (PKR)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA520Ra01Polyclonal Antibody to Protein Kinase R (PKR)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Protein Kinase R (PKR)CLIA Kit Customized Service Offer
n/aELISA Kit for Protein Kinase R (PKR)ELISA Kit Customized Service Offer
  1. "Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon."Cell 62:379-390(1990) [PubMed] [Europe PMC] [Abstract]
  2. "Mechanism of interferon action: cDNA structure, expression, and regulation of the interferon-induced, RNA-dependent P1/eIF-2 alpha protein kinase from human cells."Virology 188:33-46(1992) [PubMed] [Europe PMC] [Abstract]
  3. "Mechanism of interferon action sequence of the human interferon-inducible RNA-dependent protein kinase (PKR) deduced from genomic clones."Gene 178:191-193(1996) [PubMed] [Europe PMC] [Abstract]
  4. "Structural organization of the human gene (PKR) encoding an interferon-inducible RNA-dependent protein kinase (PKR) and differences from its mouse homolog."Genomics 36:197-201(1996) [PubMed] [Europe PMC] [Abstract]
  5. "Genomic features of human PKR: alternative splicing and a polymorphic CGG repeat in the 5'-untranslated region."J. Interferon Cytokine Res. 18:609-616(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]