Matrix Metalloproteinase 9 (MMP9)

[Edit]

GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

Matrix Metalloproteinase 9 (MMP9)

MMP-9 (also known as gelatinase B) is secreted as a 92 kDa zymogen. Cleavage of pro-MMP-9 at or near residue 87 results in the active enzyme with a mass of approximately 82 kDa. MMP-9 has three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain.

Pro-MMP-9 can be activated by MMP-3 or by certain bacterial proteinases. MMP-9 is inhibited byα2-macroglobulin or by TIMP-1, which binds to pro-MMP-9 as well as to active MMP-9.Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells. Pro-MMP-9 expression is upregulated by TGF-β1, IL-1β, TGF-α, PDGF-AB,

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Hu01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
EPA553Hu51Eukaryotic Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
RPA553Hu06Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
APA553Hu01Active Matrix Metalloproteinase 9 (MMP9)Cell culture; Activity Assays; In vivo assays.
AntibodiesPAA553Hu01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
MAA553Hu22Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAA553Hu71Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB; ICC; IHC-P; IHC-F; IF; ELISA.
LAA553Hu81FITC-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)IHC;ICC;IF;WB
PAA553Hu06Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553HuELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection
SCA553HuCLIA Kit for Matrix Metalloproteinase 9 (MMP9)Chemiluminescent immunoassay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Mu01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
RPA553Mu02Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
EPA553Mu51Eukaryotic Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling.
APA553Mu01Active Matrix Metalloproteinase 9 (MMP9)Cell culture; Activity Assays; In vivo assays.
AntibodiesPAA553Mu01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
LAA553Mu71Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)IHC;WB;ELISA
PAA553Mu07Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553MuELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection
SCA553MuCLIA Kit for Matrix Metalloproteinase 9 (MMP9)Chemiluminescent immunoassay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Ra01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
EPA553Ra51Eukaryotic Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling.
AntibodiesPAA553Ra01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
LAA553Ra71Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)IHC;WB;ELISA
LAA553Ra81FITC-Linked Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)IHC;ICC;IF;WB
MAA553Ra21Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
PAA553Ra07Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553RaELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Rb01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
AntibodiesMAA553Rb21Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
PAA553Rb51Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553RbELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Ca01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA553Ca01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
MAA553Ca21Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553CaELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Po01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA553Po01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
MAA553Po21Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553PoELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Bo01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA553Bo01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
MAA553Bo21Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553BoELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Equus caballus; Equine (Horse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA553Eq01Recombinant Matrix Metalloproteinase 9 (MMP9)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAA553Eq01Polyclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
MAA553Eq21Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEA553EqELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection
HEA553EqHigh Sensitive ELISA Kit for Matrix Metalloproteinase 9 (MMP9)Enzyme-linked immunosorbent assay for Antigen Detection
  1. "SV40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages."J. Biol. Chem. 264:17213-17221(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Complete structure of the human gene for 92-kDa type IV collagenase. Divergent regulation of expression for the 92- and 72-kilodalton enzyme genes in HT-1080 cells."J. Biol. Chem. 266:16485-16490(1991) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and comparative analysis of human chromosome 20." Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Regulatory mechanism of 92 kDa type IV collagenase gene expression which is associated with invasiveness of tumor cells."Oncogene 8:395-405(1993) [PubMed] [Europe PMC] [Abstract]
  7. "Human neutrophil gelatinase: a marker for circulating blood neutrophils. Purification and quantitation by enzyme linked immunosorbent assay."Eur. J. Haematol. 49:180-191(1992) [PubMed] [Europe PMC] [Abstract]
  8. "The cytokine-protease connection: identification of a 96-kD THP-1 gelatinase and regulation by interleukin-1 and cytokine inducers."Cytokine 3:231-239(1991) [PubMed] [Europe PMC] [Abstract]
  9. "Matrix metalloproteinase 3 (stromelysin) activates the precursor for the human matrix metalloproteinase 9."J. Biol. Chem. 267:3581-3584(1992) [PubMed] [Europe PMC] [Abstract]
  10. "Matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) from HT 1080 human fibrosarcoma cells. Purification and activation of the precursor and enzymic properties."J. Biol. Chem. 267:21712-21719(1992) [PubMed] [Europe PMC] [Abstract]
  11. "Proteolytic and non-proteolytic activation of human neutrophil progelatinase B."Biochim. Biophys. Acta 1251:99-108(1995) [PubMed] [Europe PMC] [Abstract]
  12. "Purification and identification of 91-kDa neutrophil gelatinase. Release by the activating peptide interleukin-8."Eur. J. Biochem. 198:391-398(1991) [PubMed] [Europe PMC] [Abstract]
  13. "Cytokine-mediated regulation of human leukocyte gelatinases and role in arthritis."Lymphokine Cytokine Res. 10:317-324(1991) [PubMed] [Europe PMC] [Abstract]
  14. "Latent collagenase and gelatinase from human neutrophils and their activation."Matrix Suppl. 1:245-255(1992) [PubMed] [Europe PMC] [Abstract]
  15. "Purification and characterization of human 92-kDa type IV collagenase (gelatinase B)."Exp. Mol. Med. 28:161-165(1996)
  16. "Characterization of the monomeric and dimeric forms of latent and active matrix metalloproteinase-9. Differential rates for activation by stromelysin 1."J. Biol. Chem. 275:2661-2668(2000) [PubMed] [Europe PMC] [Abstract]
  17. "Salivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease."Infect. Immun. 69:1402-1408(2001) [PubMed] [Europe PMC] [Abstract]
  18. "Cleavage of metastasis suppressor gene product KiSS-1 protein/metastin by matrix metalloproteinases."Oncogene 22:4617-4626(2003) [PubMed] [Europe PMC] [Abstract]
  19. "Extracellular matrix protein 1 inhibits the activity of matrix metalloproteinase 9 through high-affinity protein/protein interactions."Exp. Dermatol. 15:300-307(2006) [PubMed] [Europe PMC] [Abstract]
  20. "A functional polymorphism in THBS2 that affects alternative splicing and MMP binding is associated with lumbar-disc herniation."Am. J. Hum. Genet. 82:1122-1129(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Mutations in MMP9 and MMP13 determine the mode of inheritance and the clinical spectrum of metaphyseal anadysplasia."Am. J. Hum. Genet. 85:168-178(2009) [PubMed] [Europe PMC] [Abstract]
  22. "The adenomatous polyposis coli-associated exchange factors Asef and Asef2 are required for adenoma formation in Apc(Min/+)mice."EMBO Rep. 10:1355-1362(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Structure of the C-terminally truncated human ProMMP9, a gelatin-binding matrix metalloproteinase."Acta Crystallogr. D 58:1182-1192(2002) [PubMed] [Europe PMC] [Abstract]
  24. "Crystal structure of human MMP9 in complex with a reverse hydroxamate inhibitor."J. Mol. Biol. 319:173-181(2002) [PubMed] [Europe PMC] [Abstract]
  25. "Structural basis of the adaptive molecular recognition by MMP9."J. Mol. Biol. 320:1065-1079(2002) [PubMed] [Europe PMC] [Abstract]
  26. "Theoretical model of human type IV collagenase precursor."Submitted (APR-2002) to the PDB data bank
  27. "Genetic variation at the matrix metalloproteinase-9 locus on chromosome 20q12.2-13.1."Hum. Genet. 105:418-423(1999) [PubMed] [Europe PMC] [Abstract]