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Perforin 1 (PRF1)

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FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein

Perforin 1 (PRF1)
CCC B317
HGNC 9360
MGI 97551
PubMed 2783486
RGD 708463
UniProt P14222
Wiki PRF1
Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Perforin is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB317Hu01 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Hu01 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
RPB317Hu02 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Hu02 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
Antibodies PAB317Hu01 Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
MAB317Hu22 Monoclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
LAB317Hu81 FITC-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IF.
LAB317Hu71 Biotin-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC.
MAB317Hu21 Monoclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
Assay Kits SEB317Hu ELISA Kit for Perforin 1 (PRF1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB317Mu01 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB317Mu02 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Mu01 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
APB317Mu02 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
Antibodies PAB317Mu01 Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
LAB317Mu81 FITC-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IF.
LAB317Mu71 Biotin-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC.
PAB317Mu02 Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
Assay Kits SEB317Mu ELISA Kit for Perforin 1 (PRF1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB317Ra01 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Ra01 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
Antibodies PAB317Ra01 Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
MAB317Ra21 Monoclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
Assay Kits SEB317Ra ELISA Kit for Perforin 1 (PRF1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Structure and function of human perforin."Nature 335:448-451(1988) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and chromosomal assignment of a human perforin (PFP) gene."Immunogenetics 30:452-457(1989) [PubMed] [Europe PMC] [Abstract]
  3. "Structure of the human perforin gene. A simple gene organization with interesting potential regulatory sequences."J. Immunol. 143:4267-4274(1989) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Target cell-induced perforin mRNA turnover in NK3.3 cells is mediated by multiple elements within the mRNA coding region."Mol. Immunol. 33:341-349(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Human autoreactive CD4+ T cell clones use perforin- or Fas/Fas ligand-mediated pathways for target cell lysis."J. Immunol. 158:2756-2761(1997) [PubMed] [Europe PMC] [Abstract]
  9. "Perforin, Fas/Fas ligand, and TNF-alpha pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL."J. Immunol. 158:5283-5291(1997) [PubMed] [Europe PMC] [Abstract]
  10. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Perforin activates clathrin- and dynamin-dependent endocytosis, which is required for plasma membrane repair and delivery of granzyme B for granzyme-mediated apoptosis."Blood 115:1582-1593(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Human perforin employs different avenues to damage membranes."J. Biol. Chem. 286:2946-2955(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Reciprocal granzyme/perforin-mediated death of human regulatory and responder T cells is regulated by interleukin-2 (IL-2)."J. Mol. Med. 88:577-588(2010) [PubMed] [Europe PMC] [Abstract]
  14. "Localization and molecular modelling of the membrane-inserted domain of the ninth component of human complement and perforin."Mol. Immunol. 27:589-602(1990) [PubMed] [Europe PMC] [Abstract]
  15. "The structural basis for membrane binding and pore formation by lymphocyte perforin."Nature 468:447-451(2010) [PubMed] [Europe PMC] [Abstract]
  16. "Perforin gene defects in familial hemophagocytic lymphohistiocytosis."Science 286:1957-1959(1999) [PubMed] [Europe PMC] [Abstract]
  17. "Spectrum of perforin gene mutations in familial hemophagocytic lymphohistiocytosis."Am. J. Hum. Genet. 68:590-597(2001) [PubMed] [Europe PMC] [Abstract]
  18. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]