Interleukin 1 Receptor Associated Kinase 4 (IRAK4)

IRAK-4 is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors.Animals without IRAK-4 are more susceptible to viruses and bacteria but completely resistant to LPS challenge.Unlike the other IRAK proteins, however, but similar to Pelle, IRAK4 has a short C-terminal domain. Northern blot analysis revealed expression of 3.0- and 4.4-kb transcripts, with strongest expression in kidney and liver. Luciferase reporter analysis showed that IRAK4 lacking the kinase domain inhibited IL1- but not tumor necrosis factor (TNF)- induced NFKB and IRAK1 activation. SDS-PAGE and autoradiographic analysis indicated that IRAK4 phosphorylates and activates IRAK1 at thr387, but not vice versa.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPB518Hu01Recombinant Interleukin 1 Receptor Associated Kinase 4 (IRAK4)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB518Hu01Polyclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4)WB, ICC, IHC-P, IHC-F, ELISA
MAB518Hu22Monoclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB518HuELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Interleukin 1 Receptor Associated Kinase 4 (IRAK4)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4)CLIA Kit Customized Service Offer
n/aELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPB518Ra01Recombinant Interleukin 1 Receptor Associated Kinase 4 (IRAK4)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB518Ra01Polyclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4)WB, ICC, IHC-P, IHC-F, ELISA
MAB518Ra21Monoclonal Antibody to Interleukin 1 Receptor Associated Kinase 4 (IRAK4)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB518RaELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4)Enzyme-linked immunosorbent assay for Antigen Detection
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  14. "IRAK-4 kinase activity is required for interleukin-1 (IL-1) receptor- and toll-like receptor 7-mediated signaling and gene expression."J. Biol. Chem. 282:13552-13560(2007) [PubMed] [Europe PMC] [Abstract]
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  16. "The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1."Biochem. J. 409:43-52(2008) [PubMed] [Europe PMC] [Abstract]
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  20. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  21. "IRAK1 and IRAK4 promote phosphorylation, ubiquitination, and degradation of MyD88 adaptor-like (Mal)."J. Biol. Chem. 285:18276-18282(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
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  24. "Natural loss-of-function mutation of myeloid differentiation protein 88 disrupts its ability to form Myddosomes."J. Biol. Chem. 286:11875-11882(2011) [PubMed] [Europe PMC] [Abstract]
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  26. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  27. "Crystal structures of IRAK-4 kinase in complex with inhibitors: a serine/threonine kinase with tyrosine as a gatekeeper."Structure 14:1835-1844(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Crystal structures of the apo and inhibited IRAK4 kinase domain."Submitted (DEC-2006) to the PDB data bank
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  30. "Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling."Nature 465:885-890(2010) [PubMed] [Europe PMC] [Abstract]
  31. "TLR9 activation induces normal neutrophil responses in a child with IRAK-4 deficiency: involvement of the direct PI3K pathway."J. Immunol. 179:4754-4765(2007) [PubMed] [Europe PMC] [Abstract]
  32. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
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  35. "Functional assessment of the mutational effects of human IRAK4 and MyD88 genes."Mol. Immunol. 58:66-76(2014) [PubMed] [Europe PMC] [Abstract]