Apolipoprotein B (APOB)

Apolipoprotein B (APOB) is the primary apolipoprotein of low-density lipoproteins (LDL or "bad cholesterol"), which is responsible for carrying cholesterol to tissues. While it is unclear exactly what functional role APOB plays in LDL, it is the primary apolipoprotein component and is absolutely required for its formation. What is clear is that the APOB on the LDL particle acts as a ligand for LDL receptors in various cells throughout the body.

Through a mechanism that is not fully understood, high levels of APOB can lead to plaques that cause vascular disease (atherosclerosis), leading to heart disease. There is considerable evidence that levels of APOB are a better indicator of heart disease risk than total cholesterol or LDL.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC003Hu01 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
NPC003Hu01 Native Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Hu02 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC003Hu01 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
MAC003Hu22 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Hu71 Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
LAC003Hu81 FITC-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IF.
MAC003Hu21 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
PAC003Hu02 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
Assay Kits SEC003Hu ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC003Hu CLIA Kit for Apolipoprotein B (APOB) Chemiluminescent immunoassay for Antigen Detection.
HEC003Hu High Sensitive ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC003Mu01 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
RPC003Mu02 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC003Mu01 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Mu71 Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
LAC003Mu81 FITC-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IF.
PAC003Mu02 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
Assay Kits SEC003Mu ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
HEC003Mu High Sensitive ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC003Ra01 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC003Ra01 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Ra71 Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
LAC003Ra81 FITC-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IF.
MAC003Ra21 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Ra72 Biotin-Linked Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
Assay Kits SEC003Ra ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC003Po01 Recombinant Apolipoprotein B (APOB) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC003Po01 Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
LAC003Po81 FITC-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IF.
LAC003Po71 Biotin-Linked Polyclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC.
MAC003Po21 Monoclonal Antibody to Apolipoprotein B (APOB) WB; IHC; ICC; IP.
Assay Kits SEC003Po ELISA Kit for Apolipoprotein B (APOB) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Complete cDNA and derived protein sequence of human apolipoprotein B-100."Nucleic Acids Res. 14:7501-7503(1986) [PubMed] [Europe PMC] [Abstract]
  2. "DNA sequence of the human apolipoprotein B gene."DNA 6:363-372(1987) [PubMed] [Europe PMC] [Abstract]
  3. "The complete cDNA and amino acid sequence of human apolipoprotein B-100."J. Biol. Chem. 261:12918-12921(1986) [PubMed] [Europe PMC] [Abstract]
  4. "Human liver apolipoprotein B-100 cDNA: complete nucleic acid and derived amino acid sequence."Proc. Natl. Acad. Sci. U.S.A. 83:8142-8146(1986) [PubMed] [Europe PMC] [Abstract]
  5. "The complete sequence and structural analysis of human apolipoprotein B-100: relationship between apoB-100 and apoB-48 forms."EMBO J. 5:3495-3507(1986) [PubMed] [Europe PMC] [Abstract]
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Analysis of cDNA clones encoding the entire B-26 region of human apolipoprotein B."Proc. Natl. Acad. Sci. U.S.A. 83:5678-5682(1986) [PubMed] [Europe PMC] [Abstract]
  8. "Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B."Proc. Natl. Acad. Sci. U.S.A. 83:1467-1471(1986) [PubMed] [Europe PMC] [Abstract]
  9. "Isolation and characterization of sulfhydryl and disulfide peptides of human apolipoprotein B-100."Proc. Natl. Acad. Sci. U.S.A. 87:5523-5527(1990) [PubMed] [Europe PMC] [Abstract]
  10. "Human apolipoprotein B-100: cloning, analysis of liver mRNA, and assignment of the gene to chromosome 2."Proc. Natl. Acad. Sci. U.S.A. 82:8340-8344(1985) [PubMed] [Europe PMC] [Abstract]
  11. "A partial cDNA clone for human apolipoprotein B."Proc. Natl. Acad. Sci. U.S.A. 82:4983-4986(1985) [PubMed] [Europe PMC] [Abstract]
  12. "Human apolipoprotein B: partial amino acid sequence."FEBS Lett. 170:105-108(1984) [PubMed] [Europe PMC] [Abstract]
  13. "Hypobetalipoproteinemia due to an apolipoprotein B gene exon 21 deletion derived by Alu-Alu recombination."J. Biol. Chem. 264:11394-11400(1989) [PubMed] [Europe PMC] [Abstract]
  14. "Analysis of the human apolipoprotein B gene; complete structure of the B-74 region."Gene 49:29-51(1986) [PubMed] [Europe PMC] [Abstract]
  15. "Structural comparison of human apolipoproteins B-48 and B-100."Biochemistry 26:5478-5486(1987) [PubMed] [Europe PMC] [Abstract]
  16. "Molecular cloning of human apolipoprotein B cDNA."Nucleic Acids Res. 13:8813-8826(1985) [PubMed] [Europe PMC] [Abstract]
  17. "A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine."Cell 50:831-840(1987) [PubMed] [Europe PMC] [Abstract]
  18. "Human apolipoprotein B (apoB) mRNA: identification of two distinct apoB mRNAs, an mRNA with the apoB-100 sequence and an apoB mRNA containing a premature in-frame translational stop codon, in both liver and intestine."Proc. Natl. Acad. Sci. U.S.A. 85:1772-1776(1988) [PubMed] [Europe PMC] [Abstract]
  19. "Carboxyl terminal analysis of human B-48 protein confirms the novel mechanism proposed for chain termination."Biochem. Biophys. Res. Commun. 149:1214-1219(1987) [PubMed] [Europe PMC] [Abstract]
  20. "Identification of a novel in-frame translational stop codon in human intestine apoB mRNA."Biochem. Biophys. Res. Commun. 148:279-285(1987) [PubMed] [Europe PMC] [Abstract]
  21. "Human apolipoprotein B: identification of cDNA clones and characterization of mRNA."Nucleic Acids Res. 13:6937-6953(1985) [PubMed] [Europe PMC] [Abstract]
  22. "Human apolipoprotein B: structure of carboxyl-terminal domains, sites of gene expression, and chromosomal localization."Science 230:37-43(1985) [PubMed] [Europe PMC] [Abstract]
  23. "Molecular cloning and expression of partial cDNAs and deduced amino acid sequence of a carboxyl-terminal fragment of human apolipoprotein B-100."Proc. Natl. Acad. Sci. U.S.A. 82:7265-7269(1985) [PubMed] [Europe PMC] [Abstract]
  24. "Molecular cloning of human LDL apolipoprotein B cDNA. Evidence for more than one gene per haploid genome."Atherosclerosis 58:277-289(1985) [PubMed] [Europe PMC] [Abstract]
  25. "Isolation, expression and characterization of a human apolipoprotein B 100-specific cDNA clone."Biol. Chem. Hoppe-Seyler 367:1077-1083(1986) [PubMed] [Europe PMC] [Abstract]
  26. "Apolipoprotein B-48 is the product of a messenger RNA with an organ-specific in-frame stop codon."Science 238:363-366(1987) [PubMed] [Europe PMC] [Abstract]
  27. "Complete protein sequence and identification of structural domains of human apolipoprotein B."Nature 323:734-738(1986) [PubMed] [Europe PMC] [Abstract]
  28. "Sequence, structure, receptor-binding domains and internal repeats of human apolipoprotein B-100."Nature 323:738-742(1986) [PubMed] [Europe PMC] [Abstract]
  29. "Apolipoprotein B is a calcium binding protein."Biochem. Biophys. Res. Commun. 137:493-499(1986) [PubMed] [Europe PMC] [Abstract]
  30. "Palmitoylation of apolipoprotein B is required for proper intracellular sorting and transport of cholesteroyl esters and triglycerides."Mol. Biol. Cell 11:721-734(2000) [PubMed] [Europe PMC] [Abstract]
  31. "Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."Proteomics 4:454-465(2004) [PubMed] [Europe PMC] [Abstract]
  32. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
  33. "Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection."Cell. Microbiol. 8:565-580(2006) [PubMed] [Europe PMC] [Abstract]
  34. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  35. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  36. "Biological, clinical and population relevance of 95 loci for blood lipids." Nature 466:707-713(2010) [PubMed] [Europe PMC] [Abstract]
  37. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  38. "A novel mutation of apolipoprotein B in a French Canadian family with homozygous hypobetalipoproteinemia."J. Clin. Lipidol. 5:414-417(2011) [PubMed] [Europe PMC] [Abstract]
  39. "Proprotein convertase subtilisin/kexin type 9 interacts with apolipoprotein B and prevents its intracellular degradation, irrespective of the low-density lipoprotein receptor."Arterioscler. Thromb. Vasc. Biol. 32:1585-1595(2012) [PubMed] [Europe PMC] [Abstract]
  40. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  41. "Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene."Hum. Genet. 86:91-93(1990) [PubMed] [Europe PMC] [Abstract]
  42. "Association between a specific apolipoprotein B mutation and familial defective apolipoprotein B-100."Proc. Natl. Acad. Sci. U.S.A. 86:587-591(1989) [PubMed] [Europe PMC] [Abstract]
  43. "Sequence polymorphism in the human apoB gene at position 8344."Nucleic Acids Res. 18:5922-5922(1990) [PubMed] [Europe PMC] [Abstract]
  44. "Familial ligand-defective apolipoprotein B. Identification of a new mutation that decreases LDL receptor binding affinity."J. Clin. Invest. 95:1225-1234(1995) [PubMed] [Europe PMC] [Abstract]
  45. "Detection of new variants in the apolipoprotein B (Apo B) gene by PCR-SSCP."Hum. Mutat. 8:282-285(1996) [PubMed] [Europe PMC] [Abstract]
  46. "Familial ligand-defective apolipoprotein B-100: simultaneous detection of the Arg3500-->Gln and Arg3531-->Cys mutations in a French population."Hum. Mutat. 10:160-163(1997) [PubMed] [Europe PMC] [Abstract]
  47. "Screening for mutations of the apolipoprotein B gene causing hypocholesterolemia."Hum. Genet. 102:44-49(1998) [PubMed] [Europe PMC] [Abstract]
  48. "A novel nontruncating APOB gene mutation, R463W, causes familial hypobetalipoproteinemia."J. Biol. Chem. 278:13442-13452(2003) [PubMed] [Europe PMC] [Abstract]
  49. "Hypobetalipoproteinemia with an apparently recessive inheritance due to a 'de novo' mutation of apolipoprotein B."Biochim. Biophys. Acta 1688:61-67(2004) [PubMed] [Europe PMC] [Abstract]
  50. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
  51. "Molecular basis of autosomal dominant hypercholesterolemia: assessment in a large cohort of hypercholesterolemic children."Circulation 123:1167-1173(2011) [PubMed] [Europe PMC] [Abstract]
  52. "Quantitative detection of single amino acid polymorphisms by targeted proteomics."J. Mol. Cell Biol. 3:309-315(2011) [PubMed] [Europe PMC] [Abstract]
  53. "Genetic variation in APOB, PCSK9, and ANGPTL3 in carriers of pathogenic autosomal dominant hypercholesterolemic mutations with unexpected low LDL-Cl Levels."Hum. Mutat. 33:448-455(2012) [PubMed] [Europe PMC] [Abstract]