High Mobility Group AT Hook Protein 1 (HMGA1)

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HMG-R; HMGIY; High mobility group protein A1; High mobility group protein R; High mobility group protein HMG-I/HMG-Y

High Mobility Group AT Hook Protein 1 (HMGA1)

High mobility group protein HMG-I/HMG-Y is  non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. HMGA1 preferentially binds to the minor groove of A T-rich regions in double-stranded DNA.

It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins.

High Mobility Group AT Hook 1 is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC050Hu01 Recombinant High Mobility Group AT Hook Protein 1 (HMGA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC050Hu01 Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IP.
MAC050Hu22 Monoclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IP.
LAC050Hu71 Biotin-Linked Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC.
LAC050Hu81 FITC-Linked Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IF.
Assay Kits SEC050Hu ELISA Kit for High Mobility Group AT Hook Protein 1 (HMGA1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC050Mu01 Recombinant High Mobility Group AT Hook Protein 1 (HMGA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC050Mu01 Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IP.
LAC050Mu71 Biotin-Linked Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for High Mobility Group AT Hook Protein 1 (HMGA1) CLIA Kit Customized Service Offer
n/a ELISA Kit for High Mobility Group AT Hook Protein 1 (HMGA1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC050Ra01 Recombinant High Mobility Group AT Hook Protein 1 (HMGA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC050Ra01 Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IP.
LAC050Ra71 Biotin-Linked Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC.
MAC050Ra21 Monoclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for High Mobility Group AT Hook Protein 1 (HMGA1) CLIA Kit Customized Service Offer
n/a ELISA Kit for High Mobility Group AT Hook Protein 1 (HMGA1) ELISA Kit Customized Service Offer

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC050Ca01 Recombinant High Mobility Group AT Hook Protein 1 (HMGA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC050Ca01 Polyclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IP.
MAC050Ca21 Monoclonal Antibody to High Mobility Group AT Hook Protein 1 (HMGA1) WB; IHC; ICC; IP.
Assay Kits SEC050Ca ELISA Kit for High Mobility Group AT Hook Protein 1 (HMGA1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Cloning of cDNAs coding for human HMG I and HMG Y proteins: both are capable of binding to the octamer sequence motif."Nucleic Acids Res. 17:5947-5959(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Alternative processing of mRNAs encoding mammalian chromosomal high-mobility-group proteins HMG-I and HMG-Y."Mol. Cell. Biol. 9:2114-2123(1989) [PubMed] [Europe PMC] [Abstract]
  3. "Organization, inducible-expression and chromosome localization of the human HMG-I(Y) nonhistone protein gene."Nucleic Acids Res. 21:4259-4267(1993) [PubMed] [Europe PMC] [Abstract]
  4. "Retinoid-dependent recruitment of a histone H1 displacement activity by retinoic acid receptor."J. Biol. Chem. 274:22563-22568(1999) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and analysis of human chromosome 6." Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The human chromosomal protein HMG I contains two identical palindrome amino acid sequences."Biochem. Biophys. Res. Commun. 146:725-730(1987) [PubMed] [Europe PMC] [Abstract]
  8. "The amino acid sequence of the chromosomal protein HMG-Y, its relation to HMG-I and possible domains for the preferential binding of the proteins to stretches of A-T base pairs."Biochem. Biophys. Res. Commun. 158:646-651(1989) [PubMed] [Europe PMC] [Abstract]
  9. "The A.T-DNA-binding domain of mammalian high mobility group I chromosomal proteins. A novel peptide motif for recognizing DNA structure."J. Biol. Chem. 265:8573-8582(1990) [PubMed] [Europe PMC] [Abstract]
  10. "Intragenic breakpoint within RAD51L1 in a t(6;14)(p21.3;q24) of a pulmonary chondroid hamartoma."Cytogenet. Cell Genet. 95:17-19(2001) [PubMed] [Europe PMC] [Abstract]
  11. "During apoptosis of tumor cells HMGA1a protein undergoes methylation: identification of the modification site by mass spectrometry."Biochemistry 42:3575-3585(2003) [PubMed] [Europe PMC] [Abstract]
  12. "Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a."Biochem. Biophys. Res. Commun. 336:831-835(2005) [PubMed] [Europe PMC] [Abstract]
  13. "Tandem mass spectrometry for the examination of the posttranslational modifications of high-mobility group A1 proteins: symmetric and asymmetric dimethylation of Arg25 in HMGA1a protein."Biochemistry 44:6293-6301(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Dynamics of human protein arginine methyltransferase 1(PRMT1) in vivo."J. Biol. Chem. 280:38005-38010(2005) [PubMed] [Europe PMC] [Abstract]
  15. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  16. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  17. "Homeodomain-interacting protein kinase-2 (HIPK2) phosphorylates HMGA1a at Ser-35, Thr-52, and Thr-77 and modulates its DNA binding affinity."J. Proteome Res. 6:4711-4719(2007) [PubMed] [Europe PMC] [Abstract]
  18. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  19. "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
  20. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  26. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  27. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  28. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  29. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  30. "The solution structure of an HMG-I(Y)-DNA complex defines a new architectural minor groove binding motif."Nat. Struct. Biol. 4:657-665(1997) [PubMed] [Europe PMC] [Abstract]