Nucleoside Phosphorylase (NP)

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PNP; PUNP; Purine Nucleoside Phosphorylase; Inosine phosphorylase; Inosine-guanosine phosphorylase

Nucleoside Phosphorylase (NP)
NP encodes the enzyme purine nucleoside phosphorylase that together with adenosine deaminase (ADA) serves a key role in purine catabolism, referred to as the salvage pathway. Mutations in either enzyme result in a severe combined immunodeficiency (SCID).
Nucleoside phosphorylase is an enzyme which, as its name implies, phosphorylates a nucleoside to produce a nucleotide monophosphate. It is one enzyme of the nucleotide salvage pathways. These pathways allow the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). Often the de novo pathway is interrupted as a result of chemotherapy drugs such as methotrexate or aminopterin.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPC683Hu01Recombinant Nucleoside Phosphorylase (NP)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAC683Hu01Polyclonal Antibody to Nucleoside Phosphorylase (NP)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Nucleoside Phosphorylase (NP)CLIA Kit Customized Service Offer
n/aELISA Kit for Nucleoside Phosphorylase (NP)ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPC683Mu01Recombinant Nucleoside Phosphorylase (NP)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAC683Mu01Polyclonal Antibody to Nucleoside Phosphorylase (NP)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Nucleoside Phosphorylase (NP)CLIA Kit Customized Service Offer
n/aELISA Kit for Nucleoside Phosphorylase (NP)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPC683Ra01Recombinant Nucleoside Phosphorylase (NP)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAC683Ra01Polyclonal Antibody to Nucleoside Phosphorylase (NP)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Nucleoside Phosphorylase (NP)CLIA Kit Customized Service Offer
n/aELISA Kit for Nucleoside Phosphorylase (NP)ELISA Kit Customized Service Offer
  1. "Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization."Nucleic Acids Res. 12:5779-5787(1984) [PubMed] [Europe PMC] [Abstract]
  2. "A human purine nucleoside phosphorylase deficiency caused by a single base change."J. Biol. Chem. 262:2332-2338(1987) [PubMed] [Europe PMC] [Abstract]
  3. "Genetic variation in genes associated with arsenic metabolism: glutathione S-transferase omega 1-1 and purine nucleoside phosphorylase polymorphisms in European and indigenous Americans."Environ. Health Perspect. 111:1421-1427(2003) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. , Submitted (FEB-2006) to UniProtKB
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  8. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  10. "Assessment of the red cell proteome of young patients with unexplained hemolytic anemia by two-dimensional differential in-gel electrophoresis (DIGE)."PLoS ONE 7:E34237-E34237(2012) [PubMed] [Europe PMC] [Abstract]
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  12. "Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2-A resolution."J. Biol. Chem. 265:1812-1820(1990) [PubMed] [Europe PMC] [Abstract]
  13. "Molecular analysis of mutations in a patient with purine nucleoside phosphorylase deficiency."Am. J. Hum. Genet. 51:763-772(1992) [PubMed] [Europe PMC] [Abstract]
  14. "Two novel missense and frameshift mutations in exons 5 and 6 of the purine nucleoside phosphorylase (PNP) gene in a severe combined immunodeficiency (SCID) patient."Hum. Genet. 98:706-709(1996) [PubMed] [Europe PMC] [Abstract]