Sphingosine Kinase 1 (SPHK1)
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1. Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor and serum.Sequence comparisons of the mammalian SPHK proteins and SPHKs from other organisms identified 5 regions that are highly conserved. Northern blot analysis of mouse adult tissues showed ubiquitous expression of Sphk1, with the highest levels found in lung and spleen.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPE698Hu01Recombinant Sphingosine Kinase 1 (SPHK1)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAE698Hu01Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)WB, ICC, IHC-P, IHC-F, ELISA
MAE698Hu21Monoclonal Antibody to Sphingosine Kinase 1 (SPHK1)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEE698HuELISA Kit for Sphingosine Kinase 1 (SPHK1)Enzyme-linked immunosorbent assay

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Sphingosine Kinase 1 (SPHK1)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Sphingosine Kinase 1 (SPHK1)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Sphingosine Kinase 1 (SPHK1)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Sphingosine Kinase 1 (SPHK1)CLIA Kit Customized Service Offer
n/aELISA Kit for Sphingosine Kinase 1 (SPHK1)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Sphingosine Kinase 1 (SPHK1)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Sphingosine Kinase 1 (SPHK1)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Sphingosine Kinase 1 (SPHK1)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Sphingosine Kinase 1 (SPHK1)CLIA Kit Customized Service Offer
n/aELISA Kit for Sphingosine Kinase 1 (SPHK1)ELISA Kit Customized Service Offer
  1. "Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution."Gene 251:19-26(2000) [PubMed] [Europe PMC] [Abstract]
  2. "Functional characterization of human sphingosine kinase-1."FEBS Lett. 473:81-84(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes."Biochem. J. 350:429-441(2000) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Cloning and characterization of a protein kinase A anchoring protein (AKAP)-related protein that interacts with and regulates sphingosine kinase 1 activity."J. Biol. Chem. 277:32947-32953(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Identification of functional nuclear export sequences in human sphingosine kinase 1."Biochem. Biophys. Res. Commun. 311:168-173(2003) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Translocation of sphingosine kinase 1 to the plasma membrane is mediated by calcium- and integrin-binding protein 1."J. Biol. Chem. 285:483-492(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2."Nature 465:1084-1088(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Molecular basis of sphingosine kinase 1 substrate recognition and catalysis."Structure 21:798-809(2013) [PubMed] [Europe PMC] [Abstract]