Eukaryotic Elongation Factor 2 Kinase (EEF2K)

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eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

Eukaryotic Elongation Factor 2 Kinase (EEF2K)
Eukaryotic elongation factor-2 kinase is a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis.The deduced 725-amino acid human protein is 90% identical to the rodent proteins. EEF2K lacks homology to other serine/threonine kinases or to other calmodulin-dependent kinases, apart from a glycine-rich loop that is part of the ATP-binding site. All EEF2K sequences contain a highly-conserved 200-residue catalytic domain. There is also a conserved C-terminal coiled-coil region. SDS-PAGE and functional analysis showed expression of a 100-kD protein whose activity was strictly calmodulin-dependent.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPF026Hu01Recombinant Eukaryotic Elongation Factor 2 Kinase (EEF2K)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAF026Hu01Polyclonal Antibody to Eukaryotic Elongation Factor 2 Kinase (EEF2K)WB, ICC, IHC-P, IHC-F, ELISA
Assay Kitsn/aCLIA Kit for Eukaryotic Elongation Factor 2 Kinase (EEF2K)CLIA Kit Customized Service Offer
n/aELISA Kit for Eukaryotic Elongation Factor 2 Kinase (EEF2K)ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Eukaryotic Elongation Factor 2 Kinase (EEF2K)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Eukaryotic Elongation Factor 2 Kinase (EEF2K)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Eukaryotic Elongation Factor 2 Kinase (EEF2K)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Eukaryotic Elongation Factor 2 Kinase (EEF2K)CLIA Kit Customized Service Offer
n/aELISA Kit for Eukaryotic Elongation Factor 2 Kinase (EEF2K)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Eukaryotic Elongation Factor 2 Kinase (EEF2K)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Eukaryotic Elongation Factor 2 Kinase (EEF2K)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Eukaryotic Elongation Factor 2 Kinase (EEF2K)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Eukaryotic Elongation Factor 2 Kinase (EEF2K)CLIA Kit Customized Service Offer
n/aELISA Kit for Eukaryotic Elongation Factor 2 Kinase (EEF2K)ELISA Kit Customized Service Offer
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  2. "The sequence and analysis of duplication-rich human chromosome 16." Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
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  5. "A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta."EMBO J. 20:4360-4369(2001) [PubMed] [Europe PMC] [Abstract]
  6. "Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase."EMBO J. 20:4370-4379(2001) [PubMed] [Europe PMC] [Abstract]
  7. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398."J. Biol. Chem. 279:12220-12231(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  10. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  11. "cdc2-cyclin B regulates eEF2 kinase activity in a cell cycle- and amino acid-dependent manner."EMBO J. 27:1005-1016(2008) [PubMed] [Europe PMC] [Abstract]
  12. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  16. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  17. "The channel-kinase TRPM7 regulates phosphorylation of the translational factor eEF2 via eEF2-k."Cell. Signal. 23:586-593(2011) [PubMed] [Europe PMC] [Abstract]
  18. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  19. "Identification of autophosphorylation sites in eukaryotic elongation factor-2 kinase."Biochem. J. 442:681-692(2012) [PubMed] [Europe PMC] [Abstract]
  20. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  21. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  22. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]