Triple Functional Domain Protein (TRIO)

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Tgat; ARHGEF23; PTPRF-interacting protein

Triple Functional Domain Protein (TRIO)
The TRIO protein contains 3 functional domains: a serine/threonine kinase domain and 2 guanine nucleotide exchange factor (GEF) domains for the family of Rho-like GTPases, specific for Rac1 and RhoA, respectively. These domains suggest that this enzyme may play a key role in several signaling pathways that control cell proliferation.
Overexpression of their N-terminal GEF domains enhanced secretion from immature granules, depleting cells of secretory cargo in the absence of secretagogue. This response required GEF activity and was mimicked by kalirin/Trio substrates Rac1 and RhoG. Selective pharmacologic inhibition of endogenous GEF activity decreased secretagogue-independent release of hormone precursors, causing accumulation of product peptide in mature secretory granules.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Triple Functional Domain Protein (TRIO)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Triple Functional Domain Protein (TRIO)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Triple Functional Domain Protein (TRIO)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Triple Functional Domain Protein (TRIO)CLIA Kit Customized Service Offer
n/aELISA Kit for Triple Functional Domain Protein (TRIO)ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Triple Functional Domain Protein (TRIO)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Triple Functional Domain Protein (TRIO)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Triple Functional Domain Protein (TRIO)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Triple Functional Domain Protein (TRIO)CLIA Kit Customized Service Offer
n/aELISA Kit for Triple Functional Domain Protein (TRIO)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Triple Functional Domain Protein (TRIO)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Triple Functional Domain Protein (TRIO)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Triple Functional Domain Protein (TRIO)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Triple Functional Domain Protein (TRIO)CLIA Kit Customized Service Offer
n/aELISA Kit for Triple Functional Domain Protein (TRIO)ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains."Proc. Natl. Acad. Sci. U.S.A. 93:5466-5471(1996) [PubMed] [Europe PMC] [Abstract]
  5. "Trio amino-terminal guanine nucleotide exchange factor domain expression promotes actin cytoskeleton reorganization, cell migration and anchorage-independent cell growth."J. Cell Sci. 112:1825-1834(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  12. "NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio."Cell 95:269-277(1998) [PubMed] [Europe PMC] [Abstract]
  13. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
  14. "Diagnostic exome sequencing in persons with severe intellectual disability."N. Engl. J. Med. 367:1921-1929(2012) [PubMed] [Europe PMC] [Abstract]