Timeless Homolog (TIM)

Cellular pacemakers located in the suprachiasmatic nucleus (SCN) of the anterior hypothalamus control circadian rhythms. In Drosophila, a central clock mechanism involves the dynamic regulation of 2 genes, 'period' (per;) and 'timeless' (tim), which physically interact and participate in an intracellular transcriptional/translational feedback loop.The predicted 1,208-amino acid human protein is 84% identical to mouse Tim. The mammalian proteins share 4 regions of homology with Drosophila tim, including regions involved in nuclear localization, protein-protein interaction with PER, and cytoplasmic localization. Northern blot analysis revealed that TIM was expressed as a 4.5-kb mRNA in all human tissues tested, with the highest levels in placenta, pancreas, thymus, and testis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Timeless Homolog (TIM) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Timeless Homolog (TIM) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Timeless Homolog (TIM) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Timeless Homolog (TIM) CLIA Kit Customized Service Offer
n/a ELISA Kit for Timeless Homolog (TIM) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Timeless Homolog (TIM) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Timeless Homolog (TIM) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Timeless Homolog (TIM) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Timeless Homolog (TIM) CLIA Kit Customized Service Offer
n/a ELISA Kit for Timeless Homolog (TIM) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Timeless Homolog (TIM) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Timeless Homolog (TIM) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Timeless Homolog (TIM) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Timeless Homolog (TIM) CLIA Kit Customized Service Offer
n/a ELISA Kit for Timeless Homolog (TIM) ELISA Kit Customized Service Offer
  1. "Identification of the mammalian homologues of the Drosophila timeless gene, Timeless1."FEBS Lett. 441:427-431(1998) [PubMed] [Europe PMC] [Abstract].
  2. "Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-ARTNL/BMAL1-induced transcription."Neuron 21:1101-1113(1998) [PubMed] [Europe PMC] [Abstract] and Skin.
  3. "Coupling of human circadian and cell cycles by the timeless protein."Mol. Cell. Biol. 25:3109-3116(2005) [PubMed] [Europe PMC] [Abstract]
  4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  5. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  6. "Tipin and Timeless form a mutually protective complex required for genotoxic stress resistance and checkpoint function."Proc. Natl. Acad. Sci. U.S.A. 103:18143-18147(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint."J. Biol. Chem. 282:2729-2740(2007) [PubMed] [Europe PMC] [Abstract]
  8. "Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors."J. Mol. Biol. 366:36-52(2007) [PubMed] [Europe PMC] [Abstract]
  9. "The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement."Mol. Cell. Biol. 27:3131-3142(2007) [PubMed] [Europe PMC] [Abstract]
  10. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  13. "The many facets of the Tim-Tipin protein families' roles in chromosome biology."Cell Cycle 9:700-705(2010) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  15. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  16. "Timeless makes some time for itself."Cell Cycle 10:2254-2254(2011) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Mammalian TIMELESS is involved in period determination and DNA damage-dependent phase advancing of the circadian clock."PLoS ONE 8:E56623-E56623(2013) [PubMed] [Europe PMC] [Abstract]
  19. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]