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| Product: | Anti-Insulin Like Growth Factor 1 (IGF1) Monoclonal Antibody |
| Target molecule: | Insulin Like Growth Factor 1 (IGF1) |
| Host: | Mouse |
| Source: | Ascites |
| Purification: | Affinity Chromatography |
| Isotype: | IgG2b, Kappa |
| Traits: | Liquid |
| Concentration: | 500µg/mL |
| Buffer: | 0.01M PBS, pH7.4, containing 0.05% proclin-300, 50% glycerol. |
| Pairing: | RSA050Hu21 (capture antibody) is recommended to pair with RSA050Hu22 (detection antibody) in immunoassay. |
| Applications: | Recommended use: Recommended to be used in immunoassays for recognition of human IGF1. Tested application: Capture antibody (1-4ug/mL) in ELISA assay. Detection antibody (0.5-10ug/mL) in WB. Detection antibody (5-40ug/mL) in IHC. |
| Storage: | Aliquot and store at -20°C for 12 months. Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. |
Identification of Specificity:
Figure 1. ELISA
Purified antigen and natural samples were tested in ELISA with paring antibodies of RSA050Hu21 and RSA050Hu22.
Figure 2. WB
| Sample: | A. Recombinant Human IGF1 | |
| B. Lane 1: Mouse Testis Tissue | Lane2: Mouse Uterus Tissue |
(Human IGF1 has several isforms by alternative splicing.)
Figure 3. IHC
| Sample: | Human Liver Tissue |