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Active Myostatin (MSTN)

GDF8; Growth Differentiation Factor 8

ACTIVITY TEST

Myostatin (MSTN) also known as growth differentiation factor 8(GDF-8) is a member of the TGF beta protein family. Myostatin is aa secreted growth differentiation factor that produced and released by myocytes. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Besides, Bone Morphogenetic Protein 1 (BMP1) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MSTN and recombinant human BMP1. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of MSTN and BMP1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Clinical Medicine and Diagnostics Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats Sapub:Source
Nutr Metab (Lond) Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice PubMed: 26539241
International Journal of Cancer Tumor inoculation site affects the development of cancer cachexia and muscle wasting PubMed: 26016447
Connect Tissue Res Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts PubMed: 25748814
PLoS One Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease Pubmed:26998756
Calcif Tissue Int. The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats Pubmed:27272029
Archives of medical research Irisin and Myostatin Levels in Patients with Graves' Disease. pubmed:27986127
Calcified Tissue International Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats pubmed:29242963
Scientific Reports Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. pubmed:28740214
Journal of Animal Science Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs Pubmed: 30203098
European Journal of Internal Medicine Predictive value of serum myostatin for the severity and clinical outcome of heart failure Pubmed: 31056368
J Cell Physiol Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats Pubmed: 31960436
NEPHROLOGY DIALYSIS TRANSPLANTATION Myostatin and muscle atrophy during chronic kidney disease Pubmed: 32974666
Oxid Med Cell Longev Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance … 34868459
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