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Active Fatty Acid Binding Protein 4 (FABP4)

A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein

ACTIVITY TEST

Fatty acid binding protein-4 (FABP4) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner. FABP4 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length. Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Peroxisome Proliferator Activated Receptor Gamma (PPARg) has been identified as an interactor of FABP4, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FABP4 and recombinant human PPARg. Briefly, FABP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PPARg-coated microtiter wells and incubated for 1h at 37℃. Wells were aspirated and incubated for 1h with anti-FABP4 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse FABP4 and recombinant human PPARg was shown in Figure 1, and this effect was in a dose dependent manner. The EC50 for this effect is 36.6 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Clinical and Molecular Hepatology Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH PubMed: 23091808
journal of Human Hypertension Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults PubMed: 26134622
genetics and molecular research Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice PubMed: 25867423
Food Chem Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes PubMed: 25952883
Placenta Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia Pubmed:26992681
Clin Chim Acta. Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies. pubmed:28161274
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871
Nutrition & Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans Pubmed:29335416
American Journal of Cancer Research 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4 Pubmed: 31720079
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074
cells The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure Pubmed:35455943
Bone Research FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis Pubmed:35729106
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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KSB693Mu01 ELISA Kit DIY Materials for Fatty Acid Binding Protein 4 (FABP4) Main materials for "Do It(ELISA Kit) Yourself"