Active Tubulin Beta (TUBb)

TUB-B; TUBB5; TUBB1; M40

ACTIVITY TEST

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin Beta (TUBb) is a spherical molecule which is composed of 455 amino acids. Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) has a remarkably high affinity for TUBb, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TUBb and recombinant human YWHAz. Briefly, TUBb was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to YWHAz-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TUBb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human TUBb and recombinant human YWHAz was shown in Figure 1, the EC50 for this effect is 0.88 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
archives of pharmacal research Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues pubmed:27747473
archiv der pharmazie (Weinheim). New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. pubmed:28150327
ACS Applied Materials & Interfaces Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture pubmed:28112513
Future Medicinal Chemistry Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents Pubmed:29779400
Future Medicinal Chemistry Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization Pubmed:29966433
Journal of Biological Chemistry Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids Pubmed: 30237167
Journal of Enzyme Inhibition and Medicinal Chemistry Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors Pubmed: 30191744
European Journal of Medicinal Chemistry Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study Pubmed: 31536891
ACS Sensors Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform Pubmed: 32207606
Bioorganic Chemistry Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their … Pubmed: 32325332
BRITISH JOURNAL OF NUTRITION Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats Pubmed: 33028437
European Journal of Medicinal Chemistry Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon … Pubmed: 33158579
J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells 33300466
Cancer Cell International Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
Cancer Cell Int Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212
Sci Rep Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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MAB870Hu21 Monoclonal Antibody to Tubulin Beta (TUBb) WB; IHC; ICC; IP.
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