Active Fibroblast Growth Factor 21 (FGF21)

ACTIVITY TEST

Fibroblast growth factor 21(FGF21) is a protein that inmammals is encoded by the FGF21 gene. The proteinencoded by this gene is a member of the fibroblastgrowth factor (FGF) family and specifically a memberof the endocrine subfamily which includes FGF23 andFGF15/19. FGF21 is the primary endogenous agonist ofthe FGF21 receptor, which is composed of the co-receptors FGF receptor 1 and β-Klotho. Besides, Klotho Beta (KLb) has been identified as an interactor of FGF21, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF21 and recombinant human KLb. Briefly, FGF21 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl then transferred to KLb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KLb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of KLb and FGF21 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Lipids in Health and Disease Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs Lipidworld: source
Endocrinology Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source
Biochemical and Biophysical Research Communications Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH Pubmed:25449271
Free Radic Biol Med Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway PubMed: 25843656
Journal of diabetes and its complications Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. pubmed:27916484
American journal of the medical sciences Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus pubmed:29078839
Frontiers in Physiology Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555
Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим …
Physiol Res Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. Pubmed: 32469231
Chinese Journal of Osteoporosis 2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究.
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats 33833309
Nutrients The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose
Medicine (Baltimore) Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort 34918690
Annals of Agricultural and Environmental Medicine Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis 34969228
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … Pubmed:35212074
Lab Invest Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury Pubmed:35488034
Molecular Biology Reports Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC918Hu01 Recombinant Fibroblast Growth Factor 21 (FGF21) Positive Control; Immunogen; SDS-PAGE; WB.
APC918Hu61 Active Fibroblast Growth Factor 21 (FGF21) Cell culture; Activity Assays.
EPC918Hu61 Eukaryotic Fibroblast Growth Factor 21 (FGF21) Positive Control; Immunogen; SDS-PAGE; WB.
APC918Hu01 Active Fibroblast Growth Factor 21 (FGF21) Cell culture; Activity Assays.
PAC918Hu01 Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
FAC918Hu01 Anti-Fibroblast Growth Factor 21 (FGF21) Polyclonal Antibody Flow cytometry.
MAC918Hu21 Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
MAC918Hu23 Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
MAC918Hu24 Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
MAC918Hu22 Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
MAC918Hu25 Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
MAC918Hu27 Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21) WB; IHC; ICC; IP.
SEC918Hu ELISA Kit for Fibroblast Growth Factor 21 (FGF21) Enzyme-linked immunosorbent assay for Antigen Detection.
MEC918Hu Mini Samples ELISA Kit for Fibroblast Growth Factor 21 (FGF21) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC918Hu CLIA Kit for Fibroblast Growth Factor 21 (FGF21) Chemiluminescent immunoassay for Antigen Detection.
CCC918Hu CLIA Kit for Fibroblast Growth Factor 21 (FGF21) Chemiluminescent immunoassay for Antigen Detection.
LMC918Hu Multiplex Assay Kit for Fibroblast Growth Factor 21 (FGF21) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.