≡

Active Klotho (KL)

KLa; Klotho Alpha

ACTIVITY TEST

Klotho (KL) is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. The Klotho protein is a novel β-glucuronidase capable of hydrolyzing steroid β-glucuronides. Genetic variants in KLOTHO have been associated with human aging, and Klotho protein has been shown to be a circulating factor detectable in serum that declines with age. The binding of certain fibroblast growth factors (FGF's) viz., FGF19, FGF20, and FGF23, to their fibroblast growth factor receptors, is promoted via their interactions as co-receptors with β-Klotho. Besides, Fibroblast Growth Factor 23 (FGF23) has been identified as an interactor of KL, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat KL and recombinant rat FGF23. Briefly, KL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FGF23-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-KL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of KL and FGF23 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of KL with FGF23.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Life Sciences High phosphate feeding induced arterial medial calcification in uremic rats: Roles of Lanthanum carbonate on protecting vasculature Pubmed: 24012609
EXPERIMENTAL & CLINICAL CARDIOLOGY Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice Com:Source
Int J Clin Exp Pathol.? α-Klotho is an acute phase protein and altered by restraint stress in mice Pubmed:Pmc4203207
Annales de Biologie Clinique Les biomarqueurs des calcifications vasculaires PubMed: 26069069
Mol Med Rep Effect of secondary hyperparathyroidism serum on endothelial cells and intervention with Klotho PubMed: 25873020
Calcif Tissue Int Human Preosteoblastic Cell Culture from a Patient with Severe Tumoral Calcinosis-Hyperphosphatemia Due to a NewGALNT3 PubMed: 25899975
中华内分泌代谢杂志 抗衰老蛋白Klotho 阻断高糖培养的大鼠肾小球系膜细胞TLR4/NF-κB p65/NGAL 通路的激活及其作用机制 Cn: Article
Mol Cell Endocrinol The function of miR-199a-5p/Klotho regulating TLR4/NF-κB p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism PubMed: 26419931
Arch Med Sci Soluble Klotho protein as a novel serum biomarker in patients with acromegaly pmc:PMC4754356
Am J Nephrol Klotho Deficiency Aggravates Tacrolimus-Induced Renal Injury via the Phosphatidylinositol 3-Kinase-Akt-Forkhead Box Protein O Pathway Pubmed:27174564
Sci Rep. Klotho preservation via histone deacetylase inhibition attenuates chronic kidney disease-associated bone injury in mice. pubmed:28387374
Nephrology Is there a role for newer biomarkers in chronic kidney disease-mineral and bone disorder management? pubmed:28429560
Journal of Integrative Nephrology & Andrology Associations between serum fibroblast growth factor 23 level and intradialytic hypotension in hemodialysis patients issn:2394-2916
International Journal of Clinical and Experimental Pathology Protective effect of restructuring klotho protein combined with trimetazidine on myocardial injury in mice ISSN:1936-2625
Journal of veterinary medical science Changes of Klotho protein and Klotho mRNA expression in a hydroxy-L-proline induced hyperoxaluric rat model pubmed:28943597
Cellular Physiology and Biochemistry Klotho Reduces Necroptosis by Targeting Oxidative Stress Involved in Renal Ischemic-Reperfusion Injury Pubmed:29550818
American Journal of Translational Research Advanced maternal age impairs spatial learning capacity in young adult mouse offspring Pubmed:29636887
Journal of Diabetes and its Complications Clinical implication of alterations in serum Klotho levels in patients with type 2 diabetes mellitus and its associated complications Pubmed:30042059
Medical Science Monitor Klotho Inhibits Proliferation and Migration of Angiotensin II-Induced Vascular Smooth Muscle Cells (VSMCs) by Modulating NF-κB p65, Akt, and Extracellular Signal … Pubmed:30004089
Nephrology Dialysis Transplantation High-serum phosphate and parathyroid hormone distinctly regulate bone loss and vascular calcification in experimental chronic kidney disease Pubmed: 30189026
BMC Nephrology The role of klotho in chronic kidney disease Pubmed: 30348110
Nature Communications Age-related declines in α-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration Pubmed: 30451844
Kidney International Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome Pubmed: 30770217
Expert Opinion on Therapeutic Targets The use of LP533401 as a therapeutic option for renal osteodystrophy affects, renal calcium handling, vitamin D metabolism and bone health in uremic rats Pubmed: 30801205
Mol Immunol CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223
Journal of Clinical Hypertension Effects of salt intervention on serum levels of Klotho influenced by salt sensitivity Pubmed: 33164306
CURRENT EYE RESEARCH Expression of α-Klotho is downregulated and associated with oxidative stress in the lens in streptozotocin-induced diabetic rats Pubmed: 32744464
international immunopharmacology Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats Pubmed: 32438076
Purinergic Signalling P2X7 siRNA targeted to the kidneys increases klotho and delays the progression of experimental diabetic nephropathy. Pubmed: 32377919
Oxidative Medicine and Cellular Longevity Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury Pubmed: 33123314
NEUROSCIENCE Increased Plasma Level of Longevity Protein Klotho as a Potential Indicator of Cognitive Function Preservation in Patients With Schizophrenia Pubmed: 32612508
Pharmacological Reports ¦ÁKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy 33675964
J Mol Neurosci Investigation of the Role of Induced Overexpression of the Isolated Secreted Klotho on the A-172 Human Glioblastoma Cells Pubmed:35112226
Reproductive Biology and Endocrinology Expression of Klotho in Follicles of Patients With Polycystic Ovary Syndrome and Its Clinical Significance
Journal of Ovarian Research Klotho is a prognostic indicator of oocytes qualities in polycystic ovary syndrome
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APH757Ra01 Active Klotho (KL) Cell culture; Activity Assays.
RPH757Ra02 Recombinant Klotho (KL) Positive Control; Immunogen; SDS-PAGE; WB.
RPH757Ra01 Recombinant Klotho (KL) Positive Control; Immunogen; SDS-PAGE; WB.
PAH757Ra02 Polyclonal Antibody to Klotho (KL) WB; IHC; ICC; IP.
PAH757Ra01 Polyclonal Antibody to Klotho (KL) WB; IHC; ICC; IP.
MAH757Ra21 Monoclonal Antibody to Klotho (KL) WB; IHC; ICC; IP.
SEH757Ra ELISA Kit for Klotho (KL) Enzyme-linked immunosorbent assay for Antigen Detection.
LMH757Ra Multiplex Assay Kit for Klotho (KL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.