High Sensitive ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Stromal Cell Derived Factor 1 (SDF1).
No significant cross-reactivity or interference between High Sensitive Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant High Sensitive Stromal Cell Derived Factor 1 (SDF1) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Stromal Cell Derived Factor 1 (SDF1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 78-97 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Stromal Cell Derived Factor 1 (SDF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-101% 85-94% 80-98% 82-101%
EDTA plasma(n=5) 80-99% 83-90% 80-96% 90-98%
heparin plasma(n=5) 89-96% 82-104% 86-96% 87-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Critical Care Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD) Goescholar: source
22 The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels ScienceDirect: S0142961209012654
22 The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms ScienceDirect: S0142961211011422
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Graefe's Archive for Clinical and Experimental Ophthalmology Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells PubMed: 21655971
Molecular Therapy Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia PubMed: 21224834
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
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International Journal of Cardiology Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men Pubmed:24286591
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Pharmacological Reports The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders Pubmed:25560580
In Vitro Cellular &amp; Developmental Biology - Animal Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells Pubmed:25636237
Pharmacological Reports Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder PubMed: 26481549
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Am J Transl Res Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats PubMed: 26396666
Medicine (Baltimore) A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion PubMed: 26632727
Pharmacological Reports A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity Pubmed:27208878
Frontiers in Psychiatry Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. pubmed:28149283
European Journal of Applied Physiology High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia. pubmed:27761657
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs pubmed:29233725
International Journal of Clinical and Experimental Pathology Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424
BioMed Research International. c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer pubmed:28573141
Scientific Reports Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms. pubmed:28842629
Biomaterials Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study. pubmed:29017079
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ACS Applied Materials & Interfaces Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone Regeneration Pubmed: 30777748
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Stem Cells International Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells Pubmed: 31827537
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Acta Biomater Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia 33878473
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