High Sensitive ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)
NT-Pro-BNP; ; N-BNP
- Product No.HEA485Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range24.69-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 9.17pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 752
For more details, please contact local distributors! US$ 3384
For more details, please contact local distributors! US$ 6392
For more details, please contact local distributors! US$ 52640
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP).
No significant cross-reactivity or interference between High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Eur Rev Med Pharmacol Sci.||The role of serum n-terminal pro-brain natriuretic peptide in transient tachypnea of the newborn Europeanreview: Source|
|Transplantation Proceedings||Vascular Adhesion Protein-1, a Novel Molecule, in Kidney and Heart Allograft Recipients Pubmed: 23769096|
|Eur Heart J.||Paradoxical low-flow, low-gradient aortic stenosis despite preserved left ventricular ejection fraction: new insights from weights of operatively excised aortic valves Pubmed: 24755006|
|Integrative Medicine Insights||A Randomized Controlled Trial to Study the Effect of Yoga Therapy on Cardiac Function and N Terminal Pro BNP in Heart Failure NCBI: PMC3981569|
|Liver Int.?||Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation. Pubmed:24636620|
|Eur J Heart Fail.||Growth-differentiation factor 15 and osteoprotegerin in acute myocardial infarction complicated by cardiogenic shock: a biomarker substudy of the IABP-SHOCK II-trial. Pubmed:24903195|
|Scientific Reports||Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension PubMed: 25747153|
|Int J Mol Sci||The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension PubMed: 25984599|
|scientific reports||Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus Spironolactone PubMed: 26477337|
|Liver Int||Diabetes enhances the intrahepatic vascular response to endothelin‐1 in cirrhotic rats: association with the ETA receptor and pERK up‐regulation PubMed: 24636620|
|Int J Clin Exp Pathol||Qiliqiangxin improves cardiac function and attenuates cardiac remodeling in rats with experimental myocardial infarction PubMed: 26261541|
|Iranian Journal of Pediatrics||NT-proBNP in Children With Left to Right Shunt and Dilated Cardiomyopathy serve:4485|
|International Journal of Cardiology||Human myoblast transplantation in mice infarcted heart alters the expression profile of cardiac genes associated with left ventricle remodeling Pubmed:26457413|
|IJC Heart & Vasculature||Cardiovascular changes in patients with non-severe Plasmodium vivax malaria article:S2352-9067(16)30010-0|
|Heart Lung Circ||Soluble Glycoprotein 130 and Heat Shock Protein 27 as Novel Candidate Biomarkers of Chronic Heart Failure with Preserved Ejection Fraction Pubmed:27067668|
|International journal of cardiology||Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535|
|Experimental and Therapeutic Medicine||Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure. pubmed:28565777|
|Journal of Cardiovascular Electrophysiology||Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. pubmed:28127817|
|Journal of Cardiovascular Ecography||Study of Vitamin D Status in Patients with Dilated Cardiomyopathy at a Teaching Hospital in North India. pubmed:28465969|
|International Journal of Cardiology||5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - apreliminary approach to clinical utility. pubmed:27526355|
|Cardiovasc Toxicol||Beneficial Effects of Ozone Therapy on Oxidative Stress, Cardiac Functions and Clinical Findings in Patients with Heart Failure Reduced Ejection Fraction pubmed:28097518|
|Sci Rep.||Serial heart rhythm complexity changes in patients with anterior wall ST segment elevation myocardial infarction. pubmed:28252107|
|Journal of Cardiovascular Magnetic Resonance||The predictive capabilities of a novel cardiovascular magnetic resonance derived marker of cardiopulmonary reserve on established prognostic surrogate markers in patients with pulmonary vascular disease: results of a longitudinal pilot study pubmed:28065166|
|Repositório Institucional UNESP||AVALIAÇÃO ELETROCARDIOGRÁFICA AMBULATORIAL DE CÃES COM EHRLIQUIOSE MONOCÍTICA CRÔNICA handle:11449|
|Archivos de medicina veterinaria||Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004|
|Original article||Evaluation of N-Terminal Pro Brain Natriuretic Peptide as a biomarker for clinical severity of heart failure in pediatric population Attach432|
|Evidence-Based Complementary and Alternative Medicine||Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway pubmed:28698735|
|Scientific Reports||QSKL protects against myocardial apoptosis on heart failure via PI3K/Akt-p53 signaling pathway. pubmed:29209026|
|Vascular Pharmacology||Reversal effects of low-dose imatinib compared with sunitinib on monocrotaline-induced pulmonary and right ventricular remodeling in rats pubmed:29100963|
|Evidence-based complementary and alternative medicine||Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats pubmed:28706558|
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