High Sensitive ELISA Kit for Elastin (ELN)
WBS, WS, SVAS; Tropoelastin; Supravalvular Aortic Stenosis; Williams-Beuren Syndrome
- Product No.HEB337Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.47-30ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.19ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of High Sensitive Elastin (ELN).
No significant cross-reactivity or interference between High Sensitive Elastin (ELN) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant High Sensitive Elastin (ELN) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Elastin (ELN) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Elastin (ELN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Elastin (ELN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Elastin (ELN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Clinical & Experimental Immunology||Induction of elastin expression in vascular endothelial cells relates to hepatoportal sclerosis in idiopathic portal hypertension: possible link to serum anti-endothelial cell antibodies PubMed: 22288597|
|Stem Cell Research & Therapy||Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. Pubmed: 24739658|
|Journal of Photochemistry and Photobiology B: Biology||A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins Pubmed:25732262|
|PLoS One.||Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592|
|Nutrition & Diabetes||Effects of Exendin-4 on human adipose tissue inflammation and ECM remodelling. pubmed:27941938|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB337Hu01||Recombinant Elastin (ELN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CPB337Hu21||OVA Conjugated Elastin (ELN)||Immunogen; SDS-PAGE; WB.|
|CPB337Hu31||KLH Conjugated Elastin (ELN)||Immunogen; SDS-PAGE; WB.|
|PAB337Hu01||Polyclonal Antibody to Elastin (ELN)||WB; ICC; IHC-P; IHC-F; ELISA.|
|PAB337Hu08||Polyclonal Antibody to Elastin (ELN)||WB; IHC; ICC; IP.|
|LAB337Hu81||FITC-Linked Polyclonal Antibody to Elastin (ELN)||WB; IHC; ICC; IF.|
|LAB337Hu71||Biotin-Linked Polyclonal Antibody to Elastin (ELN)||WB; IHC; ICC.|
|MAB337Hu21||Monoclonal Antibody to Elastin (ELN)||WB; IHC; ICC; IP.|
|HEB337Hu||High Sensitive ELISA Kit for Elastin (ELN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEB337Hu||ELISA Kit for Elastin (ELN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB337Hu01||ELISA Kit DIY Materials for Elastin (ELN)||Main materials for "Do It (ELISA Kit) Yourself".|