Instant ELISA Kit for Epinephrine (EPI)

Adrenaline

Specificity

This assay has high sensitivity and excellent specificity for detection of Instant Epinephrine (EPI).
No significant cross-reactivity or interference between Instant Epinephrine (EPI) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Instant Epinephrine (EPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Epinephrine (EPI) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-97 93
EDTA plasma(n=5) 84-93 89
heparin plasma(n=5) 87-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Epinephrine (EPI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Epinephrine (EPI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Epinephrine (EPI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 80-95% 84-104% 87-94%
EDTA plasma(n=5) 78-103% 79-90% 93-101% 79-95%
heparin plasma(n=5) 99-105% 78-98% 86-94% 99-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 5 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
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Autonomic Neuroscience Study of baicalin on sympathoexcitation induced by myocardial ischemia via P2X3 receptor in superior cervical ganglia Pubmed:25554221
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735
PLoS One Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria Pubmed:27166674
Scientific Reports Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity pubmed:27694818
International Journal of Nursing Practice Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial Pubmed:29512230
Serbian Journal of Experimental and Clinical Research Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance
Frontiers in Endocrinology RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis Pubmed: 32328034
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis Pubmed: 33177714
Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair …
Anti-stress activity of some plants extracts of the North Caucasus flora
Mol Metab Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D 33549845
CRYOBIOLOGY O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress 33651993
Hypertension Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy … Pubmed:35189708
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