ELISA Kit DIY Materials for Angiogenin (ANG)

RNASE5; Ribonuclease,RNase A Family 5

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Angiogenin (ANG). No significant cross-reactivity or interference between Angiogenin (ANG) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Inflammopharmacology Evening primrose oil and celecoxib inhibited pathological angiogenesis, inflammation, and oxidative stress in adjuvant-induced arthritis: novel role of angiopoietin-1 Pubmed: 24664592
Cancer Cell Angiogenin/Ribonuclease 5 is an EGFR ligand and a serum biomarker for erlotinib sensitivity in pancreatic cancer Pubmed:29606349
Journal of Immunotherapy The VE-PTP Inhibitor AKB-9778 Improves Antitumor Activity and Diminishes the Toxicity of Interleukin 2 (IL-2) Administration Pubmed: 31348125
Clinical Hemorheology and Microcirculation Nonselective alpha-/beta-AR antagonists can inhibit pericyte proliferation, migration, and secretion in vitro Pubmed: 32224529
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KSA007Po01 ELISA Kit DIY Materials for Angiogenin (ANG) Main materials for "Do It(ELISA Kit) Yourself"