ELISA Kit DIY Materials for Glial Fibrillary Acidic Protein (GFAP)

Intermediate Filament Protein

  • Product No.KSA068Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids。
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Glial Fibrillary Acidic Protein (GFAP). No significant cross-reactivity or interference between Glial Fibrillary Acidic Protein (GFAP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Occupational Health Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain Pubmed: 23183024
Morfovirtual 2014 EFECTO PROTECTOR DEL TRATAMIENTO CON NEUROEPO SOBRE LA GLIA DE LA CORTEZA CEREBRAL EN RATONES TRANSGéNICOS CON LA ENFERMEDAD DE ALZHEIMER. Sld:Source
Annals of Anatomy Effect of memantine, a NMDA receptor blocker, on ethambutol-induced retinal injury PubMed: 26704355
Mediators of Inflammation IL-4 Inhibits IL-1β-Induced Depressive-Like Behavior and Central Neurotransmitter Alterations PubMed: 26417153
Neurochem Res Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat PubMed: 26700436
Cell Mol Neurobiol Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease PubMed: 26119304
Sleep and Biological Rhythms Relationship of orexin (hypocretin) system and astrocyte activation in Parkinson's disease with hypersomnolence Doi: 10.1111
Cellular and Molecular Neurobiology Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease Pubmed:26119304
Ann Anat Effect of memantine: A NMDA receptor blocker, on ethambutol-induced retinal injury Pubmed:26704355
United States Patent Application ESTROGENIC COMPONENTS FOR USE IN THE TREATMENT OF NEUROLOGICAL DISORDERS y2016:0101116.html
Clinical Research Human plasma glial fibrillary acidic protein and heat shock protein 27 concentrations in acute moderate-intensity aerobic exercise with different duration a825e1e27b0221df9a627769c0f614432e92.pdf
Medical Journal of Indonesia Comparison of GFAP and HSP27 concentrations in acute moderate-intensity aerobic exercise of different duration 2031884
Peptides. Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. pubmed:27777064
european journal of pharmacology Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. pubmed:27915041
european journal of pharmacology Troxerutin exerts neuroprotection in 6-hydroxydopamine lesion rat model of Parkinson's disease: Possible involvement of PI3K/ERβ signaling. pubmed:28284752
biomedicine & pharmacotherapy Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. pubmed:28199883
J Endocrinol.  Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study. pubmed:27799463
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease
Tierärztliche Hochschule Hannover Evaluierung von Biomarkern im Liquor cerebrospinalis bei paraplegischen Hunden mit Rückenmarksverletzung :
Nigerian Journal of Clinical Practice Utilization of Glial Fibrillary Acidic Protein and Galectin-3 in the Diagnosis of Cerebral Infarction Patients with Normal Cranial Tomography pubmed:28406123
Cytokine Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects pubmed:29102164
Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms pubmed:28061403
Experimental gerontology The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling. pubmed:29107062
Pesticide Biochemistry and Physiology Acrolein-mediated neurotoxicity in growing Wistar male rats Pubmed:30033014
Journal of Perinatal Medicine Mechanisms of death in structurally normal stillbirths Pubmed: 30231013
Metabolic Brain Disease Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms Pubmed: 30456649
Metabolic Brain Disease Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms Pubmed: 30421246
博士论文 INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA
Medicina The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …
Open Access Macedonian Journal of Medical Sciences Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy Pubmed: 32165929
NEUROPSYCHOBIOLOGY Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms Pubmed: 32526752
JAMA Surgery Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment Pubmed: 28593326
Acta Histochemica Quercetin mitigates monosodium glutamate-induced excitotoxicity of the spinal cord motoneurons in aged rats via p38 MAPK inhibition Pubmed: 32622428
stanbul Med J The Predictive Role of Neurobiochemical Markers in Multiple Sclerosis.
Basic and Clinical Neuroscience Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease 33995926
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KSA068Hu01 ELISA Kit DIY Materials for Glial Fibrillary Acidic Protein (GFAP) Main materials for "Do It(ELISA Kit) Yourself"