ELISA Kit DIY Materials for Leptin (LEP)
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
- Product No.KSA084Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.058ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- UOM 96T*596T*10 96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 1404
For more details, please contact local distributors! US$ 2527
For more details, please contact local distributors! US$ 4914
For more details, please contact local distributors! US$ 8424
For more details, please contact local distributors!
The Abs in the kit have high sensitivity and excellent specificity for detection of Leptin (LEP).No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|European Journal of Pharmacology||Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961|
|Indian Journal of Pharmacology||Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats Ijp: source|
|Journal of endocrinology||Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit PubMed: 22010203|
|PLoS One.||Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity PubMed: 23342013|
|Molecular and Cellular Endocrinology||Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194|
|Journal of Animal and Feed Sciences||Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores Ifzz: Source|
|British Journal of Nutrition||n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577|
|Int J Mol Sci.||Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781|
|Endocr J.||CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658|
|Animal Physiology||EFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLS Mendelu:Source|
|Evid Based Complement Alternat Med.||Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645|
|general endocrinology||Oxytocin reverse ovariectomy-induced Osteoper and body fat gain Pubmed:24506069|
|Respiratory Physiology & Neurobiology||Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring Pubmed:25500158|
|Sci Rep||Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430|
|Evid Based Complement Alternat Med||Tinospora crispa PubMed: 25821506|
|Ankara||Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilers Dergiler: 11|
|MEDICINA VETERIN||Comparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scores Scielo: S0102-09352015000300707|
|Pancreatic Disorders & Therapy||Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children Journals: 2165-7092-%20S5-00|
|Veterinary Word||Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212|
|Animal||Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. Pubmed:27074715|
|ANNALS OF ANIMAL SCIENCE||Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87|
|Annals of Animal Science||Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87|
|Obes Surg.||Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175|
|Acta Histochemica||Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234|
|J Biomed Mater Res A||Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice Pubmed:26850371|
|Clin Epigenetics.||The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model Pubmed:27110299|
|International Journal of Molecular Sciences||Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency pubmed:27690005|
|Journal of Animal Science||Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency publications:jas|
|International Journal of Endocrinology||The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162|
|journal of animal science||The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. pubmed:27482668|
|journal of animal science||Diet-induced modifications to milk composition have long-term effects on offspring growth in rabbits. pubmed:28380600|
|Evidence-Based Complementary and Alternative Medicine||Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535|
|Asian-Australasian Journal of Animal Sciences||Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817|
|Stem Cell Research & Therapy||Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. pubmed:27530249|
|Obesity Surgery||Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. pubmed:27185175|
|Meat Sci||Estimation of the intramuscular fat content of m. longissimus thoracis in crossbred beef cattle based on live animal measurements. pubmed:27940421|
|THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY||Correlation of Leptin, Insulin and Glucose During Late Gestation and Early Lactation in Murrah Buffaloes 7086|
|Journal of Animal and Plant Sciences||Effects Of Maca (Lepıdıum Meyenıı Walp) Powder On Serum Indıces And Metabolıc Responses In Racehorses 57cf073008ae83b37462323a.pdf|
|Biological Trace Element Research||Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007|
|Revista Brasileira de Ciência Avícola||Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285|
|Applied Animal Behaviour Science||Evaluating measures of exploratory behaviour in sows around farrowing and during lactation− A pilot study 10.1016/j.jacl.2017.03.007|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|APA084Hu01||Active Leptin (LEP)||Cell culture; Activity Assays.|
|RPA084Hu01||Recombinant Leptin (LEP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA084Hu01||Polyclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IP.|
|LAA084Hu71||Biotin-Linked Polyclonal Antibody to Leptin (LEP)||WB; IHC; ICC.|
|LAA084Hu81||FITC-Linked Polyclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IF.|
|MAA084Hu22||Monoclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IP.|
|MAA084Hu21||Monoclonal Antibody to Leptin (LEP)||WB; IHC; ICC; IP.|
|SEA084Hu||ELISA Kit for Leptin (LEP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA084Hu||CLIA Kit for Leptin (LEP)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA084Hu01||ELISA Kit DIY Materials for Leptin (LEP)||Main materials for "Do It (ELISA Kit) Yourself".|