ELISA Kit DIY Materials for Transforming Growth Factor Beta 1 (TGFb1)

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Cardiovascular Research Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway OxfordJournals: source
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Biochemical and Biophysical Research Communications Chronic angiotensin-(1–7) administration improves vascular remodeling after angioplasty through the regulation of the TGF-β/Smad signaling pathway in rabbits ScienceDirect: S0006291X09017021
European Cells and Materials Incorporating pTGF-β1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: efficient, sustained gene delivery to stem cells for chondrogenic differentiation. Pubmed: 22314694
Journal of Animal and Veterinary Advances A Soluble Antigen-Specific Factor from CD4+CD25+ T Cells of OVA Tolerant Mice Inducing OVA-Specific Peripheral Tolerance Independently in vivo Medwell: 2011642650
revue de medecine veterinaire Increased circulating concentrations of PDGF-BB and TGF-β1 in canine generalised demodicosis Revmedvet: Source
Biomedical and Environmental Sciences The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats Besjournal: Source
The Open Respiratory Medicine Journal Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
J Physiol Pharmacol. Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet Pubmed: 23568967
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
Journal of ethnopharmacology Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats. Pubmed: 24211397
Int J Clin Exp Pathol Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of esenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration Ijcep:Source
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Exp Ther Med. PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis Pubmed:Pmc4079423
International Journal of Molecular Medicine Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats Pubmed:25524396
Indian J Pharmacol Effect of coenzyme Q10 alone and its combination with metformin on streptozotocin-nicotinamide-induced diabetic nephropathy in rats Pubmed:25538335
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Anadolu Kardiyol Derg. A novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes. Pubmed:25592103
Journal of Drug Targeting Apoptosis, Advanced Glycation End Products and Transforming Growth Factor-β1 by Biguanides and Resveratrol to Ameliorate Experimentally-induced Diabetic Nephropathy Pdf: Drugs
Mol Med Rep NIM811 downregulates transforming growth factor PubMed: 26573209
The Tohoku Journal of Experimental Medicine Salvia Miltiorrhiza Bge.f.alba Ameliorates the Progression of Monocrotaline-Induced Pulmonary Hypertension by Protecting Endothelial Injury in Rats PubMed: 26074502
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Journal of Neuroscience MSX3 Switches Microglia Polarization and Protects from Inflammation-Induced Demyelination PubMed: 25904788
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells PubMed: 25799173
Nutr Cancer Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-4 Infected Patients in Egypt PubMed: 26492130
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Int J Clin Exp Pathol Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration. PubMed: 25972996
International Immunopharmacology Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways Pubmed:27179304
Deletion Polymorphism and Chronic Obstructive Pulmonary Disease ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells Pubmed:27075970
Tissue and Cell Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects Pubmed:27233913
Cancer Medicine & Anti Cancer Drugs Ameliorative Potential of Tamoxifen/Thymoquinone Combination in Patients with Breast Cancer: A Biochemical and Immunohistochemical Study open-access:1000102
Sleep Sciences Th17 Cell Related Cytokine Profiles in Narcolepsy and Other Types of Excessive Daytime Sleepiness article:15
Tissue Cell. Ameliorative potential of fluoxetine/raloxifene combination on experimentally induced breast cancer Pubmed:26881735
International Journal of Molecular Sciences TGF-β and Physiological Root Resorption of Deciduous Teeth. pubmed:28035998
BMC Veterinary Research Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600
immunology letters Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila. pubmed:28366526
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Journal of Cardiovascular Electrophysiology Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. pubmed:28127817
Molecular Medicine Reports The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. pubmed:27484042
International Immunopharmacology Amelioration of bleomycin-induced lung fibrosis in rats by valproic acid and butyrate: Role of nuclear factor kappa-B, proinflammatory cytokines and oxidative stress pubmed:27526269
Cellular Physiology and Biochemistry Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy pubmed:27889753
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PeerJ Platelet-rich concentrate in serum free medium enhances osteogenic differentiation of bone marrow-derived human mesenchymal stromal cells pubmed:27651984
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Research in Veterinary Science The effect of feed supplementation with effective microorganisms(EM) on pro- and anti-inflammatory cytokine concentrations in pigs pubmed:28549299
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Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111
Experiental And Therapeutic Medicine Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription pubmed:28672924
Oncotarget. Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer pubmed:29245941
International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder P-ISSN: 2349–8528
Scientific RepoRts Follistatin like-1 aggravates silicainduced mouse lung injury pubmed:28341862
Evidence-Based Complementary and Alternative Medicine Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway pubmed:28698735
Molecular Medicine Reports Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression 10.3892/mmr.2017.7563
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY  Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724
Cell Physiol Biochem Exendin-4 Induces Bone Marrow Stromal Cells Migration Through Bone Marrow-Derived Macrophages Polarization via PKA-STAT3 Signaling Pathway pubmed:29216639
Chemico-biological Interactions The promising effect of linagliptin and/or indole-3-carbinol on experimentally-induced polycystic ovarian syndrome. pubmed:28619389
PLoS One Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats pubmed:28622357
Evidence-based complementary and alternative medicine Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats pubmed:28706558
Molecular and Cellular Neuroscience TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway 10.1016/j.mcn.2017.08.006
Journal of Thoracic Oncology Systemic and Tumor Th1 and Th2 Inflammatory Profile and Macrophages in Lung Cancer: Influence of Underlying Chronic Respiratory Disease. pubmed:27793775
Journal of Controlled Release Nose-to-brain delivery of insulin enhanced by a nanogel carrier Pubmed:29196041
Journal of Cellular And Molecular Medicine  Lipopolysaccharide enhances TGF‐β1 signalling pathway and rat pancreatic fibrosis Pubmed:29424488
International Journal of Biochemistry & Cell Biology Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling Pubmed:29940125
Physiology & Behavior  Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … Pubmed:29410018
Journal of Molecular Neuroscience A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related Enzymes Pubmed:30062438
Oncotarget Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy Pubmed:29423092
Oncotarget Hypoxia-inducible factor-1α activates transforming growth factor-β1/Smad signaling and increases collagen deposition in dermal fibroblasts Pubmed:29423039
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