ELISA Kit DIY Materials for Alpha-Fetoprotein (aFP)

A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (aFP).No significant cross-reactivity or interference between Alpha-Fetoprotein (aFP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
BioFactors Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761
Food and Nutrition Sciences Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. Scirp: 5684
Diabetes Research and Clinical Practice Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats ScienceDirect: S0168822711000520
Life Sciences Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700
Annals of Hepatology Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. Hepatology: source
Biomedicine & Aging Pathology Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats ScienceDirect: S2210522013000348
European journal of pharmacology Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413
Asian Pac J Cancer Prev. Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529
Journal of Analytical Atomic Spectrometry Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source
PLOS ONE Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma Pubmed:24927126
BMC Med Imaging Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma PubMed: 25981587
Tumour Biol Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma PubMed: 26643893
Acta Veterinaria Brno The concentrations of selected blood serum proteins in calves during the first three months of life 85:33
Hepatobiliary Pancreat Dis Int Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml
Journal of Applied Pharmaceutical Science Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf
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