ELISA Kit DIY Materials for Interferon Beta (IFNb)
IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast
- Product No.KSA222Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range7.8-500pg/mL
- Applicable Sensitivity2.7pg/mL
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 1680
For more details, please contact local distributors! US$ 3024
For more details, please contact local distributors! US$ 5880
For more details, please contact local distributors! US$ 10080
For more details, please contact local distributors!
The Abs in the kit have high sensitivity and excellent specificity for detection of Interferon Beta (IFNb). No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Journal of Pineal Research||Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway PubMed: 21355876|
|Journal of General Virology||Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. Sgm: 9182080|
|Biochemical and Biophysical Research Communications||Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage ScienceDirect: S0006291X09019822|
|PLoS One||Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys PubMed: PMC3757038|
|PLoS Neglected Tropical Diseases||Innate Immune Response to Rift Valley Fever Virus in Goats PubMed: PMC3335883|
|BioMed Research International||Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages Pubmed: 24288685|
|Vet Microbiol.||An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. Pubmed: 23850441|
|Food and Chemical Toxicology||Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice Pubmed: 23535186|
|Veterinary immunology and immunopathology||Experimental model of equine alveolar macrophage stimulation with TLR ligands Pubmed: 23815824|
|American journal of respiratory cell and molecular biology||Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages Pubmed: 24502337|
|FEBS Lett.||Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity Pubmed:24530528|
|Veterinary Research||Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750|
|PLOS Neglected Tropical Diseases||MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production Pubmed:25122182|
|Veterinary Research||The innate immune response of equine bronchial epithelial cells is altered by training Pubmed:25595212|
|molecular and celluar biology||SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity PubMed: 25939384|
|Arch Virol||Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus PubMed: 25797196|
|J||Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells PubMed: 26258404|
|Virus||PKR activation enhances replication of classical swine fever virus in PK-15 cells PubMed: 25899421|
|PLoS One||siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455|
|Scientific Reports||Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 Pubmed:27090571|
|Front Microbiol||Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo Pubmed:27252695|
|Canadian Journal of Physiology and Pharmacology||Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. pubmed:28134560|
|International Journal of Molecular Medicine||Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function pubmed:27176664|
|International Journal of Clinical and Experimental Pathology||Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf|
|Poultry Science||ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages pubmed:27486255|
|Journal of Virology||Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:|
|Biochemical and Biophysical Research Communications||MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
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|KSA222Hu01||ELISA Kit DIY Materials for Interferon Beta (IFNb)||Main materials for "Do It(ELISA Kit) Yourself"|