ELISA Kit DIY Materials for Interferon Beta (IFNb)

IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interferon Beta (IFNb). No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Reference
Journal of Pineal Research Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway PubMed: 21355876
Journal of General Virology Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. Sgm: 9182080
Biochemical and Biophysical Research Communications Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage ScienceDirect: S0006291X09019822
PLoS One Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys PubMed: PMC3757038
PLoS Neglected Tropical Diseases Innate Immune Response to Rift Valley Fever Virus in Goats PubMed: PMC3335883
BioMed Research International Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages Pubmed: 24288685
Vet Microbiol. An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. Pubmed: 23850441
Food and Chemical Toxicology Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice Pubmed: 23535186
Veterinary immunology and immunopathology Experimental model of equine alveolar macrophage stimulation with TLR ligands Pubmed: 23815824
American journal of respiratory cell and molecular biology Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages Pubmed: 24502337
FEBS Lett. Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity Pubmed:24530528
Veterinary Research Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750
PLOS Neglected Tropical Diseases MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production Pubmed:25122182
Veterinary Research The innate immune response of equine bronchial epithelial cells is altered by training Pubmed:25595212
molecular and celluar biology SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity PubMed: 25939384
Arch Virol Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus PubMed: 25797196
J Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells PubMed: 26258404
Virus PKR activation enhances replication of classical swine fever virus in PK-15 cells PubMed: 25899421
PLoS One siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455
Scientific Reports Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 Pubmed:27090571
Front Microbiol Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo Pubmed:27252695
Canadian Journal of Physiology and Pharmacology Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. pubmed:28134560
International Journal of Molecular Medicine Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function pubmed:27176664
International Journal of Clinical and Experimental Pathology Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf
Poultry Science ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages pubmed:27486255
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:
Biochemical and Biophysical Research Communications MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861
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