ELISA Kit DIY Materials for Histone H3 (H3)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Histone H3 (H3). No significant cross-reactivity or interference between Histone H3 (H3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Biomed Res Int A Novel Anti-Histone H1 Monoclonal Antibody, SSV Monoclonal Antibody, Improves Lung Injury and Survival in a Mouse Model of Lipopolysaccharide-Induced Sepsis-Like Syndrome PubMed: 25649890
Cell Death & Disease Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA285Hu01 Recombinant Histone H3 (H3) Positive Control; Immunogen; SDS-PAGE; WB.
CAA285Hu01 Anti-Histone H3 (H3) Polyclonal Antibody Loading Control of WB.
PAA285Hu01 Polyclonal Antibody to Histone H3 (H3) WB; IHC; ICC; IP.
CAA285Hu22 Anti-Histone H3 (H3) Monoclonal Antibody Loading Control of WB.
KSA285Hu01 ELISA Kit DIY Materials for Histone H3 (H3) Main materials for "Do It (ELISA Kit) Yourself".