ELISA Kit DIY Materials for Cardiac Troponin I (cTnI)

TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Troponin I Type 3, Cardiac (TNNI3). No significant cross-reactivity or interference between Troponin I Type 3, Cardiac (TNNI3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Animal and Feed Sciences, The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats Pl:Source
Interact Cardiovasc Thorac Surg. Comparison of femoral and aortic remote ischaemia preconditioning for cardioprotection against myocardial ischaemia/reperfusion injury in a rat model Pubmed:25205781
Exp Ther Med Cardioprotective effect of remote preconditioning of trauma and remote ischemia preconditioning in a rat model of myocardial ischemia/reperfusion injury PubMed: 26136887
Heart International Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction pubmed:27924211
Archivos de medicina veterinaria Cardiac biomarkers in dogs with visceral leishmaniasis S0301-732X2016000300004
PLOS Pathogens Cathepsin B aggravates coxsackievirus B3-induced myocarditis through activating the inflammasome and promoting pyroptosis Pubmed:29360865
Science of the Total Environment Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and Pubmed:29727991
Molecules Melatonin balance the autophagy and apoptosis by regulating UCP2 in the LPS-induced cardiomyopathy Pubmed:29547569
Molecules The Multivariate Regression Statistics Strategy to Investigate Content-Effect Correlation of Multiple Components in Traditional Chinese Medicine Based on a … Pubmed:29494523
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781
Biological & Pharmaceutical Bulletin Protective Effects of Shenfu Injection against Myocardial Ischemia-Reperfusion Injury via Activation of eNOS in Rats Pubmed:29910216
Oncology Letters Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of … Pubmed:29928398
BMC Pharmacology and Toxicology Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis Pubmed:29321061
FASEB Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922
Frontiers in Pharmacology Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway Pubmed: 30890937
canadian journal of physiology and pharmacology Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? Pubmed: 30840489
Journal of Cellular Physiology Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes Pubmed: 30982981
BIOSENS BIOELECTRON Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA … Pubmed: 30954926
Life Sciences Protective effects of two novel nitronyl nitroxide radicals on heart failure induced by hypobaric hypoxia Pubmed: 31102744
Mikrochim Acta DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label Pubmed: 31123904
Biosensors and Bioelectronics DNA nanotetrahedron-assisted electrochemical aptasensor for cardiac troponin I detection based on the co-catalysis of hybrid nanozyme, natural enzyme and artificial …
Biofactors MiR‐183‐5p protects rat hearts against myocardial ischemia/reperfusion injury through targeting VDAC1 Pubmed: 31618500
Nature Communications Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb Pubmed: 32161256
BIOMEDICINE & PHARMACOTHERAPY Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress Pubmed: 33022535
Pharmacol Res Therapeutic potential of cPLA2 inhibitor to counteract dilated-cardiomyopathy in cholesterol-treated H9C2 cardiomyocyte and MUNO rat Pubmed: 32942017
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Early Elevation of Systemic Plasma Clusterin after Reperfused Acute Myocardial Infarction in a Preclinical Porcine Model of Ischemic Heart Disease Pubmed: 32605184
Eur J Pharmacol NLRX1 knockout aggravates lipopolysaccharide (LPS)-induced heart injury and attenuates the anti-LPS cardioprotective effect of CYP2J2/11, 12-EET by enhancing … Pubmed: 32574674
American Journal of Translational Research Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and … Pubmed: 32509190
Arabian Journal for Science and Engineering Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats
Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes
Hum Exp Toxicol Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats 33882726
Curr Protoc Isolation and Culture of Neonatal Murine Primary Cardiomyocytes 34289259
Cell Metabolism Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species 34536344
Biomed Pharmacother Pea (Pisum sativum) peel extract attenuates DOX-induced oxidative myocardial injury 34649330
Anal Bioanal Chem Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications 34673993
Biomed Res Int Nicotinic Acid Riboside Regulates Nrf-2/P62-Related Oxidative Stress and Autophagy to Attenuate Doxorubicin-Induced Cardiomyocyte Injury Pubmed:35242876
Talanta A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein Pubmed:35714415
Nutrients Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 Pubmed:35215383
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