ELISA Kit DIY Materials for Cytokeratin 5 (CK5)

KRT5; DDD; EBS2; K5; KRT5A; KRT5-A; Keratin 5; 58 kDa cytokeratin; Type-II keratin Kb5; Epidermolysis Bullosa Simplex Dowling-Meara/Kobner/Weber-Cockayne Types

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Keratin 5 (KRT5). No significant cross-reactivity or interference between Keratin 5 (KRT5) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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