ELISA Kit DIY Materials for Fatty Acid Binding Protein 2, Intestinal (FABP2)

FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 2, Intestinal (FABP2).No significant cross-reactivity or interference between Fatty Acid Binding Protein 2, Intestinal (FABP2) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Food Chem Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes PubMed: 25952883
Oxid Med Cell Longev Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model PubMed: 26064429
Vet Immunol Immunopathol Immunomodulatory potential of β-glucan as supportive treatment in porcine rotavirus enteritis. pubmed:28895864
PLoS One Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats pubmed:28622357
Journal of Immunology and Immunopathology Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function 10.5958/0973-9149.2017.00011.9
Cellular Physiology and Biochemistry Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … Pubmed:29672286
European Journal of Clinical Nutrition Lack of liver steatosis in germ-free mice following hypercaloric diets Pubmed:29926176
Nutrients Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron Pubmed:29861488
International Surgery Journal Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemia Doi: 10.18203/2349-2902.isj20190058
Hepatology Communications Effect of a Multistrain Probiotic on Cognitive Function and Risk of Falls in Patients With Cirrhosis: A Randomized Trial
Tropical Animal Health and Production Immunomodulating dose of levamisole stimulates innate immune response and prevents intestinal damage in porcine rotavirus diarrhea: a restricted-randomized … Pubmed: 30790158
Biological & Pharmaceutical Bulletin Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis Pubmed: 31582652
International Journal of Medical Sciences FABP1 and FABP2 as markers of diabetic nephropathy Pubmed: 32922200
Int Immunopharmacol Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response Pubmed: 32361189
Journal of Exercise Physiology Online Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period.
The Journal of Nutritional Biochemistry Effects of fucoidan on tumor prevention and gut flora
european journal of clinical nutrition? Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury 33514869
J Cell Mol Med The ¦Á2AR/Caveolin©\1/p38MAPK/NF©\¦ÊB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia©\reperfusion 34114328
BMC Gastroenterol Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity 34022802
JOURNAL OF APPLIED PHYSIOLOGY Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice 34528459
PLoS One The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsis Pubmed:35576220
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