ELISA Kit DIY Materials for Adiponectin (ADPN)
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
- Product No.KSA605Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range78-5,000pg/mL
- Applicable Sensitivity
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 1404
For more details, please contact local distributors! US$ 2527
For more details, please contact local distributors! US$ 4914
For more details, please contact local distributors! US$ 8424
For more details, please contact local distributors!
The Abs in the kit have high sensitivity and excellent specificity for detection of Adiponectin (ADP).No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|European Journal of Pharmacology||Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961|
|BioMed Research International||Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats PubMed: PMC3591142|
|PLoS One||CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies PubMed: PMC3297623|
|Molecular and Cellular Endocrinology||Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194|
|British Journal of Nutrition||n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577|
|International Urology Nephrology||Perirenal fat associated with microalbuminuria in obese rats Pubmed: 24526332|
|Int J Mol Sci.||Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781|
|Mol Biol Rep.||Adiponectin decreases lipids deposition by p38 MAPK/ATF2 signaling pathway in muscle of broilers. Pubmed: 24178343|
|Endocr J.||CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658|
|Iran J Basic Med Sci||Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234|
|Biometals.?||Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243|
|Poult Sci.?||Effect of perches on liver health of hens Pubmed:24812236|
|Gen Comp Endocrinol.||The effect of recombinant adeno-associated virus-adiponectin (rAAV2/1-Acrp30) on glycolipid dysmetabolism and liver morphology in diabetic rats Pubmed:25019654|
|Journal of Trace Elements in Medicine and Biology||Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats Pubmed:25194956|
|J Endocrinol||Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats Pubmed:25240061|
|Obesity Surgery||Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126|
|Int J Clin Exp Pathol||Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway PubMed: 26617802|
|Cell Physiol Biochem||Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway PubMed: 26160485|
|Life Sciences||Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet PubMed: 26188592|
|MEDICAL RESEARCH JOURNAL||Relation between afamin level and some inflammatory markers in obese children Mrjnrc: Abstract|
|Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes. Pubmed:26896925|
|Technologia Alimentaria||Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats abstract:10|
|Obes Surg.||Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175|
|Acta Histochemica||Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234|
|Adv Biomed Res.||Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393|
|Biological Trace Element Research||Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats article:s12011-016-0777-1|
|Journal of Functional Foods||Antiobesity and antihyperlipidaemic effects of Yan-Sheng-Yin in animals and humans science:S1756464616300585|
|J Bone Miner Res||Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082|
|Journal of Molecular and Cellular Cardiology||Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193|
|Journal of Diabetes Research||Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose pubmed:27127795|
|Asian-Australasian Journal of Animal Sciences||Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817|
|Biomedical and Pharmacology Journal||Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- … vol10no1|
|IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES||Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats pubmed:29147484|
|Evidence-Based Complementary and Alternative Medicine||BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway journals:1758383|
|Oxidative Medicine and Cellular Longevity||Differential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesis journals:3405089|
|Iranian Journal of Pharmaceutical Research||Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance pubmed:29201096|
|Biomedical & Pharmacology Journal||Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study DOI:10.13005/bpj/1123|
|Oncotarget||FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA605Mu01||Recombinant Adiponectin (ADPN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA605Mu02||Recombinant Adiponectin (ADPN)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA605Mu02||Polyclonal Antibody to Adiponectin (ADPN)||WB; IHC; ICC; IP.|
|PAA605Mu01||Polyclonal Antibody to Adiponectin (ADPN)||WB; IHC; ICC; IP.|
|SEA605Mu||ELISA Kit for Adiponectin (ADPN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA605Mu||CLIA Kit for Adiponectin (ADPN)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA605Mu01||ELISA Kit DIY Materials for Adiponectin (ADPN)||Main materials for "Do It (ELISA Kit) Yourself".|