ELISA Kit DIY Materials for Bradykinin (BK)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Bradykinin (BK). No significant cross-reactivity or interference between Bradykinin (BK) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Cardiology and Clinical Research Bradykinin-Induced Shock Increase Exhaled Nitric Oxide, Complement Activation and Cytokine Production in Pigs handle:10037
Journal of Allergy and Clinical Immunology A transcriptomic study of Hereditary Angioedema attacks Pubmed:29729940
Toxicology Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation Pubmed: 30685357
Thoracic Cancer Increased expression of TRPV1 in patients with acute or chronic cough after lung cancer surgery Pubmed: 30883022
Respiratory Physiology & Neurobiology Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Pubmed: 31542455
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