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ELISA Kit DIY Materials for Peroxisome Proliferator Activated Receptor Alpha (PPARa)
NR1C1; PPAR-A; HPPAR; Nuclear Receptor Subfamily 1 Group C Member 1
- Product No.KSA934Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.112ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- Downloadn/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
- FOB
US$ 1134
US$ 1890
US$ 3402
US$ 6615
US$ 11340
For more details, please contact local distributors!
SPECIFITY
The Abs in the kit have high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Alpha (PPARa). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Alpha (PPARa) and analogues was observed.
USAGE
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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| Magazine | Citations |
| Applied Physiology, Nutrition, and Metabolism | Maternal micronutrients, omega-3 fatty acids, and placental PPARγ expression Nrcresearchpress: Source |
| The Journal of Nutritional Biochemistry | Raspberry pomace alters cecal microbial activity and reduces secondary bile acids in rats fed a high-fat diet. pubmed:28437712 |
| Neuroscience Letters | Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosis pubmed:28483651 |
| Journal of Proteome Research | Proteomic investigations of transcription factors critical in geniposide-mediated suppression of alcoholic steatosis and in overdose induced hepatotoxicity on liver in … Pubmed: 31612718 |
| J Cosmet Dermatol | In©\vitro effect of pine bark extract on melanin synthesis, tyrosinase activity, production of endothelin©\1 and PPAR in cultured melanocytes exposed to Ultraviolet?¡ 33960120 |