ELISA Kit DIY Materials for Endoglin (ENG)

CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Endoglin (ENG).No significant cross-reactivity or interference between Endoglin (ENG) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Clin Rev Allergy Immunol Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature PubMed: 26142066
Angiologia e Cirurgia Vascular Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers science:S1646706X16300052
Clinical Rheumatology Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis Pubmed:26920752
Advanced Biomedical Research Diagnostic and prognostic significance of serum soluble endoglin levels in preeclampsia and eclampsia pubmed:27563629
Annals of agricultural and environmental medicine Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis. pubmed:28954507
ResearchSquare The predictive role of PlGF/sENG ratio in fetal growth restriction of early beginning associated with pre-eclampsia
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