ELISA Kit DIY Materials for Tryptase (TPS)

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Tryptase (TPS)). No significant cross-reactivity or interference between Tryptase (TPS) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Allergy and Clinical Immunology Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps PubMed: 22534535
J Pediatr. Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 Pubmed:24530123
Mediators of Inflammation Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis Pubmed:25400334
PLOS ONE A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats Pubmed:25541997
Neurogastroenterology & Motility Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery Pubmed:25677271
Mediators Inflamm Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report PubMed: 26089601
Int Immunopharmacol Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis PubMed: 26049029
Mediators of Inflammation Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins Journals: Mi
Mediators of Inflammation Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins Pubmed:27057098
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