ELISA Kit DIY Materials for Low Density Lipoprotein (LDL)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Low Density Lipoprotein (LDL). No significant cross-reactivity or interference between Low Density Lipoprotein (LDL) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
PloS one Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. NCBI: PMC3896367
PLoS One. Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL Pubmed:24465540
J Lipid Res PCSK9 deficiency unmasks a sex-and tissue-specific subcellular distribution of the LDL and VLDL receptors in mice PubMed: 26323289
Neuroscience 27-Hydroxycholesterol contributes to disruptive effects on learning and memory by modulating cholesterol metabolism in the rat brain PubMed: 25987203
The Journal of Nutritional Biochemistry Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice Pubmed:27142749
 Journal of Functional Foods Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice S1756464617300555
Int J Mol Sci. Combined Effects of Curcumin and Lycopene or Bixin in Yoghurt on Inhibition of LDL Oxidation and Increases in HDL and Paraoxonase Levels in Streptozotocin-Diabetic Rats. pubmed:28333071
Journal of Surgical Arts/Cerrahi Sanatlar Dergisi THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Hiperlipidemili ve … 163
Journal of Functional Foods Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice 10.1016:j.jff.2017.01.044
Microvascular Research Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients pubmed:28954218
Biochemical and Biophysical Research Communications Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release pubmed:29146189
Journal of Nutritional Science and Vitaminology (Tokyo) Anthocyanin Cyanidin-3-Glucoside Attenuates Platelet Granule Release in Mice Fed High-Fat Diets. pubmed:28978870
Environmental Science and Pollution Research Bifenthrin exerts proatherogenic effects via arterial accumulation of native and oxidized LDL in rats: the beneficial role of vitamin E and selenium Pubmed: 30465240
American Journal of Translational Research Alteration of circulating innate lymphoid cells in patients with atherosclerotic cerebral infarction Pubmed: 30662674
Nature Communications ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy Pubmed: 31358770
Diabetes Metab Syndr Obes ¡°Adjusting Internal Organs and Dredging Channel¡± Electroacupuncture Ameliorates Insulin Resistance in Type 2 Diabetes Mellitus by Regulating the Intestinal?¡­ 34135611
Evid Based Complement Alternat Med Effect of Acupoint Embedding on Serum Leptin and Hypothalamus Leptin Receptor Expression in Rats with Simple Obesity 34659432
Ecotoxicol Environ Saf High-fat diet-induced aggravation of cardiovascular impairment in permethrin-treated Wistar rats 34224971
Int J Food Sci Nutr Brown and golden flaxseed reduce intestinal permeability and endotoxemia, and improve the lipid profile in perimenopausal overweight women Pubmed:35311432
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