ELISA Kit DIY Materials for Interleukin 31 (IL31)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 31 (IL31). No significant cross-reactivity or interference between Interleukin 31 (IL31) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
allergy and clinical immunology Increased serum levels of Interleukin-31 could modulate inflammatory processes in multiple sclerosis Siaic-Review:Source
BMC Immunol Increased levels of interleukin 31 (IL-31) in osteoporosis PubMed: 2644965
Sci Rep. Interleukin-31 expression and relation to disease severity in human asthma pmc:PMC4783779
Exp Dermatol. Immediate-type allergic and protease-mediated reactions are involved in scratching behaviour induced by topical application of Dermatophagoides farinae extract in NC/Nga mice. pubmed:28191683
Nat Commun.  The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. pubmed:28067314
Experimental Dermatology Immediate-type allergic and protease-media ted reactions are involved in scratching behavior induced by topical application of Dermatophagoides farinae extract in NC/Nga mice doi:10.1111
アトピー性皮膚炎の痒みの発症機序に関する研究
Indian Journal of Public Health Research & Development Determination the Role of Interleukin 31 (IL-31) Levels in Three Real Allergic Diseases (Asthma, Rhinitis, Urticaria)
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
EPB179Ra61 Eukaryotic Interleukin 31 (IL31) Positive Control; Immunogen; SDS-PAGE; WB.
RPB179Ra01 Recombinant Interleukin 31 (IL31) Positive Control; Immunogen; SDS-PAGE; WB.
PAB179Ra01 Polyclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC; IP.
LAB179Ra81 FITC-Linked Polyclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC; IF.
LAB179Ra71 Biotin-Linked Polyclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC.
MAB179Ra21 Monoclonal Antibody to Interleukin 31 (IL31) WB; IHC; ICC; IP.
SEB179Ra ELISA Kit for Interleukin 31 (IL31) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB179Ra Multiplex Assay Kit for Interleukin 31 (IL31) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB179Ra01 ELISA Kit DIY Materials for Interleukin 31 (IL31) Main materials for "Do It (ELISA Kit) Yourself".