ELISA Kit DIY Materials for Ribonuclease A3 (RNASE3)

Rnase-A3; RnaseA3; ECP; RNS3; Ribonuclease,RNase A Family 3; Eosinophil Cationic Protein

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Ribonuclease A3 (RNASE3). No significant cross-reactivity or interference between Ribonuclease A3 (RNASE3) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Reference
Journal of American Science Assessment of the Role of Serum and Urine Eosinophil Cationic Protein in Diagnosis of Wuchereria bancrofti Infection Jofamerican: source
Molecular Medicine Reports Intercellular adhesion molecule?1 expression in activated eosinophils is associated with mucosal remodeling in nasal polyps Pubmed:25573100
International Journal of Molecular Sciences The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study Pubmed:26927088
annals of allergy asthma & immunology Interleukin 16 and CCL17/thymus and activation-regulated chemokine in patients with aspirin-exacerbated respiratory disease. pubmed:27986411
European Journal of Clinical Investigation Dying blood mononuclear cell secretome exerts antimicrobial activity. pubmed:27513763
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