ELISA Kit DIY Materials for Vascular Endothelial Growth Factor 121 (VEGF121)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor 121 (VEGF121). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor 121 (VEGF121) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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INCREMENT SERVICES

Magazine Citations
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
BMC Cancer VEGF-121 plasma level as biomarker for response to anti-angiogenetic therapy in recurrent glioblastoma Pubmed:29747600
JOURNAL OF CLINICAL NEUROSCIENCE A pediatric case of anaplastic astrocytoma with a gliomatosis cerebri; the growth pattern and changes in serum VEGF-121 levels after bevacizumab treatment Pubmed: 33222957
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE Associations between VEGF isoforms and impending retinopathy of prematurity Pubmed: 32737903
international journal of oncology Activin A triggers angiogenesis via regulation of VEGFA and its overexpression is associated with poor prognosis of oral squamous cell carcinoma Pubmed: 32377747
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