ELISA Kit DIY Materials for Toll Like Receptor 5 (TLR5)

CD285; SLEB1; TIL3; Toll/Interleukin-1 Receptor-Like Protein 3

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Toll Like Receptor 5 (TLR5).No significant cross-reactivity or interference between Toll Like Receptor 5 (TLR5) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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