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ELISA Kit DIY Materials for Collagen Type X (COL10)

COL10A1; COL10-A1; Schmid Metaphyseal Chondrodysplasia; Collagen Alpha-1(X)chain

  • Product No.KSC156Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.118ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Collagen Type X (COL10). No significant cross-reactivity or interference between Collagen Type X (COL10) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Tissue Eng Part A Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I PubMed: 26123891
Gene Ther rAAV-mediated overexpression of sox9, TGF-β and IGF-I in minipig bone marrow aspirates to enhance the chondrogenic processes for cartilage repair PubMed: 26583804
American Journal of Pathology Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing Pubmed: 30339839
Type X Collagen Assays and Methods of Use Thereof
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC156Hu01 Recombinant Collagen Type X (COL10) Positive Control; Immunogen; SDS-PAGE; WB.
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LAC156Hu71 Biotin-Linked Polyclonal Antibody to Collagen Type X (COL10) WB; IHC; ICC.
LAC156Hu81 FITC-Linked Polyclonal Antibody to Collagen Type X (COL10) WB; IHC; ICC; IF.
MAC156Hu22 Monoclonal Antibody to Collagen Type X (COL10) WB; IHC; ICC; IP.
SEC156Hu ELISA Kit for Collagen Type X (COL10) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC156Hu CLIA Kit for Collagen Type X (COL10) Chemiluminescent immunoassay for Antigen Detection.
LMC156Hu Multiplex Assay Kit for Collagen Type X (COL10) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC156Hu01 ELISA Kit DIY Materials for Collagen Type X (COL10) Main materials for "Do It(ELISA Kit) Yourself"