ELISA Kit DIY Materials for Neurofilament, Light Polypeptide (NEFL)

CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein

  • Product No.KSE038Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.057ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL). No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Acta Neurologica Scandinavica Effect of carotid endarterectomy on brain damage markers Pubmed:27126899
Acta Neurologica Scandinavica Effect of carotid endarterectomy on brain damage markers. pubmed:27126899
journal of neuroimmunology Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications Pubmed: 31254929
Cerebellum Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia 33893614
Medicine (Baltimore) Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis 34678870
J Clin Med Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients … Pubmed:35566558
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RPE038Hu03 Recombinant Neurofilament, Light Polypeptide (NEFL) Positive Control; Immunogen; SDS-PAGE; WB.
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KSE038Hu01 ELISA Kit DIY Materials for Neurofilament, Light Polypeptide (NEFL) Main materials for "Do It (ELISA Kit) Yourself".