ELISA Kit DIY Materials for Tumor Protein, Translationally Controlled 1 (TPT1)

HRF; TCTP; p02; p23; Fortilin; Translationally-Controlled Tumor Protein; Histamine-releasing factor

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1). No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Reference
Diabetologia Translationally controlled tumour protein is associated with podocyte hypertrophy in a mouse model of type 1 diabetes SpringerLink: e14361700w260x75
Int Arch Allergy Immunol. Synergistic Actions of Histamine-Releasing Factor and Histamine Releasing Factor-Reactive IgE in Chronic Urticaria. pubmed:28219065
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
SEG962Ra ELISA Kit for Tumor Protein, Translationally Controlled 1 (TPT1) Enzyme-linked immunosorbent assay for Antigen Detection.
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