Multiplex Assay Kit for Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay)

CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-97 93
EDTA plasma(n=5) 90-104 101
heparin plasma(n=5) 83-97 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 98-105% 85-95% 92-101%
EDTA plasma(n=5) 92-105% 92-105% 84-92% 88-102%
heparin plasma(n=5) 79-93% 81-105% 89-99% 81-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ECF) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY Elevated Serum Level of CD48 in Patients with Intermittent Allergic Rhinitis Pubmed: 32966985
Exp Mol Pathol The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­ 33285209
Immunopharmacol Immunotoxicol sCD48 is elevated in non-allergic but not in allergic persistent rhinitis 34477021
Exp Eye Res Sulforaphane inhibits cytokine-stimulated chemokine and adhesion molecule expressions in human corneal fibroblasts: Involvement of the MAPK, STAT, and NF-κB … Pubmed:35038457
Catalog No. Related products for research use of Rhesus monkey (Simian) Organism species Applications (RESEARCH USE ONLY!)
RPA025Si01 Recombinant Eosinophil Chemotactic Factor (ECF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA025Si01 Polyclonal Antibody to Eosinophil Chemotactic Factor (ECF) WB; IHC; ICC; IP.
MAA025Si21 Monoclonal Antibody to Eosinophil Chemotactic Factor (ECF) WB; IHC; ICC; IP.
SEA025Si ELISA Kit for Eosinophil Chemotactic Factor (ECF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA025Si CLIA Kit for Eosinophil Chemotactic Factor (ECF) Chemiluminescent immunoassay for Antigen Detection.
LMA025Si Multiplex Assay Kit for Eosinophil Chemotactic Factor (ECF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.