Multiplex Assay Kit for Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay)

EL; EDL; Endothelial Lipase; Endothelial cell-derived lipase

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-98 95
EDTA plasma(n=5) 95-102 99
heparin plasma(n=5) 86-95 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipase, Endothelial (LIPG) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-98% 78-105% 87-97% 92-101%
EDTA plasma(n=5) 86-102% 83-98% 95-103% 78-93%
heparin plasma(n=5) 85-97% 88-95% 79-94% 78-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:LIPG) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Journal of lipid research Lipase maturation factor 1 is required for endothelial lipase activity PubMed: 21447484
The American Journal of Clinical Nutrition Randomized controlled study of the effect of a butter naturally enriched in trans fatty acids on blood lipids in healthy women1 Ajcn: source
J Lipid Res. Effect of weight loss, independent of change in diet composition, on apolipoprotein AI kinetic in men with metabolic syndrome Pubmed: 23125458
The Korean Journal of Internal Medicine Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation Pubmed:27243230
PLoS One. Circulating C1q complement/TNF-related protein (CTRP) 1, CTRP9, CTRP12 and CTRP13 concentrations in Type 2 diabetes mellitus: In vivo regulation by glucose. pubmed:28207876
BMC Psychiatry Association of plasma endothelial lipase levels on cognitive impairment Pubmed: 31216999
biomolecules Treadmill Running Changes Endothelial Lipase Expression: Insights from Gene and Protein Analysis in Various Striated Muscle Tissues and Serum
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