Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

SPECIFITY

The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
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Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880
Arquivos Brasileiros de Oftalmologia Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitis PubMed: 26677041
Neuropeptides Melanocortin MC7 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat PubMed: 25616531
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J Pharm Pharmacol Modulation of oxidative and inflammatory cardiac response by nonselective 1‐and 4‐cyclooxygenase inhibitor and benznidazole in mice PubMed: 26105113
Theriogenology Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum PubMed: 25765299
Vet Res Commun Inflammatory cytokine and acute phase protein concentrations in the peripheral blood and uterine washings of cows with subclinical endometritis in the late postpartum period PubMed: 25846950
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удк Циток Cgi-Bin: Irbis_Nbuv
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Cell Mol Neurobiol Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease PubMed: 26119304
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The Journal of Biochemistry Role of reactive oxygen species-mediated MAPK and NF-κB activation in polygonatum cyrtonemalectin-induced apoptosis and autophagy in human lung adenocarcinoma A549 cells Pubmed:27318358
Veterinary Immunology and Immunopathology Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period Pubmed:27090626
American Journal of Translational Research Human mesenchymal stem cells attenuate experimental bronchopulmonary dysplasia induced by perinatal inflammation and hyperoxia Pubmed:27158330
Cellular and Molecular Neurobiology Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease Pubmed:26119304
Nutrition Journal Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study Pubmed:27071648
Journal of Translational Medicine Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation Pubmed:26847569
Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes. Pubmed:26896925
Advances in Experimental Medicine and Biology  Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury Pubmed:27283193
Neuroscience Bexarotene protects against traumatic brain injury in mice partially through apolipoprotein E Pubmed:27235741
Research in Veterinary Science A chimeric protein composed of the binding domains ofClostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse model Pubmed:27473983
Neurological Research: A Journal of Progress in Neurosurgery, Neurology and Neurosciences Telmisartan ameliorates oxidative stress and subarachnoid haemorrhage-induced cerebral vasospasm Pubmed:27078703
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Hepatology Research Amelioration of free fatty acid-induced fatty liver by quercetin-3-O-β-D-glucuronide through modulation ofperoxisome proliferator-activated receptor-alpha/sterol reCavia (Guinea pig )latory element-binding protein-1c signaling. Pubmed:26190035
Biomedicine & Pharmacotherapy Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate Pubmed:27261592
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Int J Clin Exp Pathol Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism files:ijcep0019305.pdf
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Mediators of Inflammation Plasma Periostin Levels Are Increased in Chinese Subjects with Obesity and Type 2 Diabetes and Are Positively Correlated with Glucose and Lipid Parameters journals:6423637
Biol Trace Elem Res. Analysis of Ionomic Profiles of Canine Hairs Exposed to Lipopolysaccharide (LPS)-Induced Stress Pubmed:26758868
Endocrine Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways Pubmed:26758998
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Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro Pubmed:26706290
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Virol J Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathway Pubmed:27296632
Clin Epigenetics. The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model Pubmed:27110299
Int J Mol Sci. Dexmedetomidine Protects Rat Liver against Ischemia-Reperfusion Injury Partly by the α2A-Adrenoceptor Subtype and the Mechanism Is Associated with the TLR4/NF-κB Pathway Pubmed:27347929
Journal of Neuroinflammation Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats pubmed:27716383
Journal of traditional and complementary medicine. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. pubmed:27774414
Front Pharmacol Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670
BMC Veterinary Research Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600
Oxidative Medicine and Cellular Longevity Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. pubmed:27656261
Journal of Pre-Clinical and Clinical Research Circulating cytokeratin-18 and tumour necrosis factor-α in patients with alcoholic liver cirrhosis 582a2e2208aef19cb8053ce8.pdf
BMC Complementary and Alternative Medicine Effect of traditional Chinese medicine formula Sinisan on chronic restraint stress-induced nonalcoholic fatty liver disease: a rat study. pubmed:28388904
immunology letters Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila. pubmed:28366526
IUBMB Life. Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status. pubmed:28067023
Lipids in Health and Disease Protective effects of various ratios of DHA/EPA supplementation on high-fat diet-induced liver damage in mice. pubmed:28356106
Journal of Functional Foods Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolism S1756464617300890
biomedicine & pharmacotherapy Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation. pubmed:28033582
Nutrition. Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. pubmed:27908549
Journal of diabetes investigation Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus. pubmed:28035763
International Journal of Molecular Medicine The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552
未查到 Clinical effect of electro-acupuncture on lung injury patients caused by severe 3162851.pdf
Journal of Molecular and Cellular Cardiology Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193
Springerplus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway pubmed:27026909
Andrology Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function through increased endothelial dysfunction, oxidative stress, apoptosis, and corporal fibrosis … doi:10.1111
Cellular Physiology and Biochemistry The Neuroprotective Effect of Puerarin in Acute Spinal Cord Injury Rats. pubmed:27576607
Journal of Orthopaedic Surgery and Research  No midterm advantages in the middle term using small intestinal submucosa and human amniotic membrane in Achilles tendon transverse tenotomy pubmed:27881176
Atherosclerosis Iridoid–loganic acid versus anthocyanins from the Cornus mas fruits (cornelian cherry): Common and different effects on diet-induced atherosclerosis, PPARs … S0021915016314046
International Journal of Clinical and Experimental Pathology Level of serum apolipoprotein m and its diagnostic significance in inflammatory bowel disease files:ijcep0033416.pdf
Molecular Medicine Reports C‑reactive protein/oxidized low density lipoprotein/β2‑glycoprotein i complexes induce lipid accumulation and inflammatory reaction in macrophages via p38/mitogen‑ … 10.3892
Physiological Research Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. pubmed:28006947
Molecular Medicine Reports Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects pubmed:27432278
The American Journal of Chinese Medicine Pinellia ternata Attenuates Mucus Secretion and Airway Inflammation after InhaledCorticosteroid Withdrawal in COPD Rats. pubmed:27430907
Cardiovascular Diabetology Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. pubmed:27809851
PLOS ONE Protective Effects of Bifidobacterium on Intestinal Barrier Function in LPS-Induced EnterocyteBarrier Injury of Caco-2 Monolayers and in a Rat NEC Model. pubmed:27551722
Food & Function Protective effects of fish oil on pre-diabetes: a lipidomic analysis of liver ceramides in rats. pubmed:27722538
PLOS ONE Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit. pubmed:27391952
The Journal of Comenius University in Bratislava Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury acm.2017.16.issue-3
Journal of Physiology and Pharmacology Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury. pubmed:28195073
Applied Biochemistry and Biotechnology Protective Effect of Zingiber officinale Against Dalton's Lymphoma Ascites Tumour byRegulating Inflammatory Mediator and Cytokines. pubmed:27435276
Future Microbiology Transplantation of a bacterial consortium ameliorates trinitrobenzenesulfonic acid-induced colitisand intestinal dysbiosis in rats. pubmed:27415182
BioMed Research International Dihydromyricetin Protects against Diabetic Cardiomyopathy in Streptozotocin-Induced DiabeticMice. pubmed:28421194
Saudi Journal of Gastroenterology Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice. pubmed:27976641
Parasites & Vectors Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. pubmed:27502394
中国药物警戒 小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响 Periodical_zgywjj201608004
Oncotarget Poly(ADP-ribose) polymerase 1 inhibition protects cardiomyocytes from inflammation andapoptosis in diabetic cardiomyopathy. pubmed:27027354
Life Sciences Bone fracture healing is delayed in splenectomic rats pubmed:27956350
Journal of Ethnopharmacology Learning and memory improvement and neuroprotection of Gardenia jasminoides (Fructusgardenia) extract on ischemic brain injury rats. pubmed:27940085
Food Funct. Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways. pubmed:28256654
 Research in Veterinary The effect of feed supplementation with zinc chelate and zinc sulphate on selected humoral and cell-mediated immune parameters and cytokine concentration in … S0034528816303162
Mol Med Rep. Galectin-3 inhibition ameliorates hypoxia-induced pulmonary artery hypertension. pubmed:27959409
Parasitology. Nonsteroidal anti-inflammatory is more effective than anti-oxidant therapy in counteracting oxidative/nitrosative stress and heart disease in T. cruzi-infected mice. pubmed:28134069
Ren Fail.  Effect of concomitant administration of coenzyme Q10 with sitagliptin on experimentally induced diabetic nephropathy in rats. pubmed:27841100
PLoS One. Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. pubmed:28207788
Sci Rep.  Comparison of human adipose stromal vascular fraction and adipose-derived mesenchymal stem cells for the attenuation of acute renal ischemia/reperfusion injury. pubmed:28276451
Cell Death Dis. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148
Journal of Photochemistry and Photobiology B: Biology Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha levels among patients with periodontal disease: A prospective randomized split-mouth clinical study. pubmed:28282558
Journal of Cellular Physiology Autophagy maintains the integrity of endothelial barrier in LPS-induced lung injury. pubmed:28328069
Key Engineering Materials The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture. 16629795
Biomedicine & pharmacotherapy= Biomedecine & pharmacotherapie Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion pubmed:28273631
Journal of Neurochemistry β‐Caryophyllene protects in vitro neurovascular unit against oxygen‐glucose deprivation and re‐oxygenation‐induced injury pubmed:27565895
Journal of Functional Foods Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis S1756464616303528
International Journal of Stem Cell Research and Transplantation Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines IJST-2328-3548-04-803
Домашня сторінка ВПЛИВ КРОВОВТРАТИ НА ДИНАМІКУ ВМІСТУ ЦИТОКІНІВ СИРОВАТКИ КРОВІ В ПЕРІОД ГОСТРОЇ РЕАКЦІЇ НА КРАНІОСКЕЛЕТНУ ТРАВМУ 83646
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA133Ra01 Active Tumor Necrosis Factor Alpha (TNFa) Cell culture; Activity Assays.
RPA133Ra01 Recombinant Tumor Necrosis Factor Alpha (TNFa) Positive Control; Immunogen; SDS-PAGE; WB.
PAA133Ra01 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) WB; IHC; ICC; IP.
LAA133Ra81 FITC-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) WB; IHC; ICC; IF.
LAA133Ra71 Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) WB; IHC; ICC.
MAA133Ra21 Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) WB; IHC; ICC; IP.
SEA133Ra ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA133Ra High Sensitive ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA133Ra CLIA Kit for Tumor Necrosis Factor Alpha (TNFa) Chemiluminescent immunoassay for Antigen Detection.
KSA133Ra01 ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa) Main materials for "Do It (ELISA Kit) Yourself".