CLIA Kit for Resistin (RETN)

ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

Specificity

This assay has high sensitivity and excellent specificity for detection of Resistin (RETN).
No significant cross-reactivity or interference between Resistin (RETN) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Resistin (RETN) and the recovery rates were calculated by comparing the measured value to the expected amount of Resistin (RETN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 86-95 90
heparin plasma(n=5) 79-99 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin (RETN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Resistin (RETN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-90% 91-103% 92-99% 84-97%
EDTA plasma(n=5) 96-104% 86-104% 83-96% 85-104%
heparin plasma(n=5) 79-94% 80-97% 80-95% 90-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645
J Endocrinol. Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle Pubmed:24681828
Life Sciences Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Pubmed:25445438
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126
Evid Based Complement Alternat Med Tinospora crispa PubMed: 25821506
Journal of Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysis PubMed: 26686707
Genome Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without Complications PubMed: 25120108
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234
Journal of Dairy Science Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow 108495429X:34
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535
Oncotarget FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway pubmed:28515350
Journal of Dairy Science Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels pubmed:28803009
Toxicology Biological effects of adipocytes in sulfur mustard induced toxicity pubmed:29129815
Breast Cancer Research Bashar Zeidan1, Antigoni Manousopoulou2, Diana J. Garay-Baquero2, 3, Cory H. White3, 7, Samantha ET Larkin1, Kathleen N. Potter1, Theodoros I … Pubmed:29566726
Bioscience Reports Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871
Oncotarget Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120
Journal of Functional Foods Mango leaf tea promotes hepatoprotective effects in obese rats Doi: 10.1016/j.jff.2018.09.010
Food Research International Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats Doi: 10.1016/j.foodres.2019.04.012
Journal of Functional Foods Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet
Nat Commun Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler Pubmed: 31092829
Nutrients Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of … Pubmed: 31514318
GYNECOLOGICAL ENDOCRINOLOGY Saliva resistin as a screening marker of gestational diabetes mellitus Pubmed: 32804001
Chinese Journal of Natural Medicines Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles Pubmed: 32768169
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body … Pubmed: 32545900
Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin … 34438908
High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
Skeletal Muscle Fatty Acid Metabolism is Diverted Towards the Synthesis of Signaling Intermediates Without Fatty Acid Uptake Contribution in Dry Cows with Obesity …
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA847Mu01 Recombinant Resistin (RETN) Positive Control; Immunogen; SDS-PAGE; WB.
RPA847Mu02 Recombinant Resistin (RETN) Positive Control; Immunogen; SDS-PAGE; WB.
PAA847Mu01 Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
PAA847Mu02 Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
LAA847Mu81 FITC-Linked Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC; IF.
LAA847Mu71 Biotin-Linked Polyclonal Antibody to Resistin (RETN) WB; IHC; ICC.
MAA847Mu21 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Mu29 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Mu22 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Mu23 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Mu24 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Mu25 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
MAA847Mu27 Monoclonal Antibody to Resistin (RETN) WB; IHC; ICC; IP.
SEA847Mu ELISA Kit for Resistin (RETN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA847Mu CLIA Kit for Resistin (RETN) Chemiluminescent immunoassay for Antigen Detection.
LMA847Mu Multiplex Assay Kit for Resistin (RETN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA847Mu01 ELISA Kit DIY Materials for Resistin (RETN) Main materials for "Do It (ELISA Kit) Yourself".